1. Forschungsseminar: Proteinherstellung und Reinigung
Seminarraum 1.1.33, Mo 10:00-12:00
2. Topics in Molecular Biophysics: Production of biological samples in biophysics
17.9.2012-21.9.2012 from 9:00 am-18:00 pm
Master students, diploma strudents and PhD students with interest in generating their biological samples by themselves for biophysical investigations.
about basics e.g. how to mutagenise a protein, cloning techniques, expression and purification of proteins and the underlying cellular processes when expressing a protein will be given prior to the practical exercises in the lab.
Practical exercises :
molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein
-cultivation of bacteria to express the protein
-purification of membrane proteins by affinity chromatography
-amplification of DNA by PCR (Polymerase chain reaction)
-analysis of DNA with enzymes and agarose-gel-electrophoresis
-analysis of proteins on SDS-gels.