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publications
2012
- B. Repen, E. Schneider, and U. Alexiev.
Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins. Anal. Biochemistry, 2012, in press.
- K. Kirchberg, H. Michel, and U. Alexiev.
Net Proton Uptake Is Preceded by Multiple Proton Transfer Steps upon Electron Injection into Cytochrome c Oxidase. J. Biol. Chem., 2012, 287, 8187-93.
- T.-Y. Kim , T. Schlieter , S. Haase, and U. Alexiev.
Activation and molecular recognition of the GPCR rhodopsin - Insights from time-resolved fluorescence depolarisation and single molecule experiments. Eur J Cell Biol., 2012, 91, 300-310.
2011
- A. Boreham, T.-Y. Kim, V. Spahn, C. Stein, L. Mundhenk, A. D. Gruber, R. Haag, P. Welker, K. Licha, and U. Alexiev.
Exploiting fluorescence lifetime plasticity in FLIM: Target molecule localisation in cells and tissues. ACS Med. Chem. Lett., 2011, 2 (10), 724–728.
- K. Kirchberg, T.-Y. Kim, M. Möller, D. Skegro, G. Dasara Raju, J. Granzin, G. Büldt, R. Schlesinger, and U. Alexiev.
Conformational dynamics of helix-8 in the GPCR rhodopsin controls arrestin activation in the desensitization process. PNAS, 2011, 108 (46), 18690-18695.
2010
- A. Dutta, K. C. Tirupula, U. Alexiev, and J. Klein-Seetharaman.
Characterization of membrane protein non-native states. 1. Extent of unfolding and aggregation of rhodopsin in the presence of chemical denaturants. Biochemistry, 2010, 49, 6317-6328.
- A. Dutta, T.-Y. Kim, M. Moeller, J. Wu, U. Alexiev, and J. Klein-Seetharaman.
Characterization of membrane protein non-native states. 2. The SDS-unfolded states of rhodopsin. Biochemistry, 2010, 49, 6329-6340.
- K. Kirchberg, T.-Y. Kim, S. Haase, and U. Alexiev.
Functional interaction structures of the photochromic retinal protein rhodopsin. Photochem. Photobiol. Sci., 2010, 9, 226-33.
2009
- T.-Y. Kim, H. Uji-i, M. Moeller, B. Muls, J. Hofkens, and U. Alexiev.
Monitoring the interaction of a single G-protein key binding site with rhodopsin disk membranes upon light-activation. Biochemistry, 2009, 48(18), 3801-3.
- T.-Y. Kim, M. Moeller, K. Winkler, K. Kirchberg, and U. Alexiev.
Dissection of environmental changes at the cytoplasmic surface of light-activated bacteriorhodopsin and visual rhodopsin: sequence of spectrally silent steps. Photochemistry and Photobiology, 2009, 85, 570–577.
- M. Moeller and U. Alexiev.
Surface Charge Changes upon Formation of the Signaling State in Visual Rhodopsin. Photochemistry and Photobiology, 2009, 85, 501–508.
2008
- D. Narzi, K. Winkler, J. Saidowsky, R. Misselwitz, A. Ziegler, R.A. Böckmann, and U. Alexiev
Molecular determinants of MHC class I complex stability: Shaping antigenic features through long-range electrostatic interactions.
J. Biol. Chem. 2008, 283, 23093-23103.
2007
- K. Winkler, A. Winter, C. Rückert, B. Uchanska-Ziegler, and U. Alexiev.
Natural MHC Class I Polymorphism Controls the Pathway of Peptide Dissociation from HLA-B27 complexes.
Biophys. J., 2007, 93(8), 2743-55.
- K. Winkler, J. Saidowsky, A. Ziegler, and U. Alexiev.
Molecular determinants of MHC class I stability.
In:International Proceedings of the 13th International Congress of Immunology (Kalil, J. et al eds.), Medimont Bologna (2007) ISBN 978-88-7587-379-0
- T.-Y. Kim, K. Winkler, and U. Alexiev.
Picosecond Multi-dimensional Fluorescence Spectroscopy: A tool to measure real-time protein dynamics during function.
Photochemistry and Photobiology 83, 378-384 (2007)
- N. Lehmann, U. Alexiev, and K. Fahmy.
Linkage Between the Intramembrane H-bond Network Around Aspartic Acid83 and the Cytosolic Environment of Helix 8 in Photoactivated Rhodopsin.
J. Mol. Biol. 366, 1129-1141 (2007)
2006
- B. Uchanska-Ziegler, U. Alexiev, R. Hillig, M. Hülsmeyer, T. Pöhlmann, W. Saenger, A. Volz, and A. Ziegler.
X-ray crystallography and dynamic studies of HLA-B*2705 and B*2709 molecules complexed with the same peptide.
In: Immunobiology of the Human MHC: Proceedings of the 13th International Histocompatibility Workshop and Conference (Hansen, J.A., ed.), IHWG Press, Seattle (2006) ISBN:0-945278-03-9
2005
- G. Schröder, U. Alexiev, and H. Grubmüller.
Simulation of fluorescence anisotropy experiments: Probing protein dynamics.
Biophys. J. 89(6), 3757-70 (2005)
- A. Selle, C. Kappelt, M. A. Bader, G. Marowsky, K. Winkler, U. and Alexiev.
Picosecond pulse induced two-photon fluorescence enhancement in biological material applying grating waveguide structures.
Optics Lett. 30, 1683-1685 (2005)
- V. Jaakola, M. Rehn, M. Möller, U. Alexiev, A. Goldman, and G. J. Turner.
G-protein-coupled receptor domain over-expression in Halobacterium salinarium: Long-range allosteric interactions from the intracellular to the extracellular side in heptahelical membrane proteins.
Proteins 60, 412-23 (2005)
- M. Hülsmeyer, K. Welfle, T. Pöhlmann, R. Misselwitz, U. Alexiev, H. Welfle, W. Saenger, B. Uchanska- Ziegler, and A. Ziegler.
Thermodynamic and structural equivalence of two HLA-B27 subtypes complexed with a Self-peptide.
J. Mol. Biol. 346, 1367-1379 (2005)
2004
- T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev.
Differential peptide dynamics is linked to MHC polymorphism.
J. Biol. Chem. 279, 28197-28201 (2004)
2003
- U. Alexiev, I. Rimke, and T. Pöhlmann.
Elucidation of the nature of the conformational changes of the EF-interhelical loop in bacteriorhodopsin and of the helix VIII on the cytoplasmic surface of rhodopsin: A time-resolved fluorescence depolarization study.
J. Mol. Biol. 328, 705-719 (2003)
- B. Uchanska-Ziegler, U. Alexiev, R. Hillig, M. Hülsmeyer, T. Pöhlmann, W. Saenger, A. Volz, and A. Ziegler (2003).
In HLA 2002. The immunobiology of the human MHC. Eds. Dupont B, Hansen J. IHWG Press, Seattle
2002
- T. Mielke, U. Alexiev, M. Gläsel, H. Otto, and M. P. Heyn.
Light-induced changes in the structure and accessibility of the cytoplasmic loops of rhodopsin in the activated MII-state.
Biochemistry 41, 7875-84 (2002)
- R. A. Krebs, U. Alexiev, R. Parthasarathy, A.-M. DeVita, and M. S. Braiman.
Detection of light-activated H+-release and M-intermediate formation from purified proteorhodopsin.
BMC Physiology 2 (2002)
2000
- U. Alexiev, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn.
Evidence for long range allosteric interactions between the extracellular and cytoplasmic parts of bacteriorhodopsin from the mutant R82A and its second site revertant R82A/G231C.
J. Biol. Chem. 275, 13431-13440 (2000)
- K. Heyne, J. Herbst, B. Dominguez Herradon, U. Alexiev, and R. Diller.
Reaction control in bacteriorhodopsin: Impact of Arg82 and Asp85 on the fast retinal isomerization, studied in the second site revertant Arg82Ala/Gly231Cys and various purple and blue forms of bacteriorhodopsin.
Phys. Chem. B 104, 6053-6058 (2000)
- M. S. Hutson, U. Alexiev , S. V. Shilov, K. J. Wise, and M. S. Braiman.
Evidence for a perturbation of Arginine-82 in the bacteriorhodopsin photocycle from Time-resolved Infrared spectra.
Biochemistry 39, 13189-13200 (2000)
1998
- W. Behrens, U. Alexiev, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn
Structure of the interhelical loops and carboxyl terminus of bacteriorhodopsin by X-ray diffraction using site-directed heavy-atom labeling.
Biochemistry 37, 10411-10419 (1998)
- K. Heyne, R. Dziewior, R. Diller, and U. Alexiev.
Single and double mutants of bacteriorhodopsin and their impact on photoisomerization.
In Springer Series in Chemical Physics: "Ultrafast phenomena XI". Eds. T. Elsaesser, J.G. Fujimoto, D. A. Wiersma, W. Zinth, 681-683 (1998)
1996
- S. Moltke, U. Alexiev, and M. P. Heyn.
Kinetics of light-induced intramolecular charge transfer and proton release in bacteriorhodopsin.
Israel. J. Chem. 35, 235-248 (1996)
1995
- U. Alexiev, R. Mollaaghababa, P. Scherrer, H. G. Khorana, and M. P. Heyn.
Rapid long range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.
PNAS 92, 372-376 (1995)
- U. Alexiev, P. Scherrer, T. Marti, H. G. Khorana, and M. P. Heyn.
Time-resolved surface charge change on the cytoplasmic side of bacteriorhodopsin.
FEBS Letters 373, 81-84 (1995)
- S. Dickopf, U. Alexiev, M. P. Krebs, H. Otto, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn.
Proton transport by a bacteriorhodopsin mutant, aspartic acid-85—> asparagine, initiated in the unprotonated Schiff base state.
PNAS 92, 11519-11523 (1995)
1994
- U. Alexiev, T. Marti, M. P. Heyn, H. G. Khorana, and P. Scherrer.
Surface charge of bacteriorhodopsin detected with covalently bound pH-indicators at selected extracellular and cytoplasmic sites.
Biochemistry 33, 298 - 306 (1994)
- P. Scherrer, U. Alexiev, T. Marti, H. G. Khorana, and M. P. Heyn.
Covalently bound pHindicator dyes at selected extracellular or cytoplasmic sites in bacteriorhodopsin. I. Lightinduced proton release kinetics and proton migration along the surface of bacteriorhodopsin micelles.
Biochemistry 33, 13684-13699 (1994)
- U. Alexiev, T. Marti, M. P. Heyn, H. G. Khorana, and P. Scherrer.
Covalently bound pHindicator dyes at selected extracellular or cytoplasmic sites in bacteriorhodopsin. II. Rotational orientation of helices D and E and kinetic correlation between M-formation and proton release in bacteriorhodopsin micelles.
Biochemistry 33, 13693-13699 (1994)
1992
- P. Scherrer, U. Alexiev, M. P. Heyn, T. Marti, and H.G. Khorana.
Proton movement and surface charge in bacteriorhodopsin detected by selectively attached pH-indicators.
In Structures and Functions of Retinal Proteins. Ed. J.L. Rigaud. Colloque INSERM/John Libbey Eurotext Ltd. Vol. 221: 205 - 211 (1992)
- D. A. Greenhalgh, S. Subramaniam, U. Alexiev, H. Otto, M. P. Heyn, and H. G. Khorana.
Effect of introducing different carboxylate-containing side chains at position 85 on chromophore formation and proton transport in bacteriorhodopsin.
J. Biol. Chem. 267, 25734-25738 (1992)
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