Hi, my name is Tom Resler, I was born on the island Rügen in the Baltic Sea in 1986. After the wall came down we moved to the island Borkum in the North Sea where I was grown up. I studied physics at the Freie Universität in Berlin and started my PhD in the group of Prof. Heberle in 2012.
The goal of my research is to achieve an atomistic understanding of the proton transfer reactions in Channelrhodopsin-2 (ChR2) and how these linked to the mechanism of ion gating. This membrane protein is a light-gated cation-selective channel from the algae Chlamydomonas Reinhardtii. It is used as an optogenetic tool to elicit action potentials by light.
Based on light-induced Fourier transform infrared (FTIR) difference spectroscopy I try to explore the kinetics on the transient changes by rapid scan and step scan technique. For band assignment different variants of ChR2 are used. Complementary I use UV/Vis spectroscopy to obtain the kinetics and to characterize the intermediate state of the photo cycle. All techniques cover the whole time range from 10 ns up to minutes. In addition pH indicating dyes are used to report proton release and uptake from the aqueous medium.