Site-directed spin labeling EPR
The course gives an introduction to electron paramagnetic resonance (EPR) spectroscopy, with special focus on site-directed spin labeling (SDSL) for the study of biomolecules. SDSL EPR is a biophysical technique providing structural and dynamic information on proteins to bridge the gap between the static snapshot of a crystal structure and the protein’s dynamic nature in a physiological environment. At the beginning of the course a brief introduction on amino acids and protein's primary-tertiary structures will be provided. The course will then start from a general description of the magnetic resonance phenomena: classical description and Bloch equations, quantum description of the spin system, spin Hamiltonian, density operators, product operator formalism (spin 1/2). The nitroxide-based spin labels available for protein studies are presented and the properties of their continuous wave EPR spectra are described in detail with focus on the dynamics and polarity effects on the spectral features and the multi-frequency advantage. Continuous wave and pulsed methods to measure interspin distances are described. A schematic description of continuous wave and pulsed spectrometers is given. State of the art applications of SDSL EPR to water soluble and membrane proteins are presented at the end of the course.
Exercises will cover the theoretical aspects presented in the course and two lab courses may be offered (to be decided with the students) on continuous wave or pulsed EPR techniques.
Lectures: Mondays from 12:00 to 14:00 in room 1.1.16
Slides and additional material will be provided during the lecture. Selected material can be also found here (registered students: please send me an e-mail and you will get access)
Introduction: why using EPR to study proteins?
||Spins of metals
Single crystal and g tensor
Direct product for coupled spins
||Nuclear isotope database (from Easyspin)
Exercises: Tuesdays from 14:00 to 16:00 in room 1.1.16. Note that on 14/10/2014 there will be no exercises. The exercises will start on 21/10/2014.
Exercises and solutions will be available here. (Registered students: please send me an e-mail and you get access)
||hand in by
| praktikum CW EPR
|| 16.12 12:00-18:00 Room 0.4.40
||06.02.2015 11:00-19:00 Room 0.4.40
if you are interested in the EPR summer school 2015: http://www.helmholtz-berlin.de/events/efepr/index_de.html
- Lehninger Principles of Biochemistry. By: David L. Nelson, Michael M. Cox. Chapters on: Amino acids, peptides and proteins & The three-dimensional structure of proteins
-"Structure validation by Calpha geometry: phi, psi and Cbeta deviation". Lovell et al. Proteins 2003, 50(3):437-50.
General Magnetic Resonance Theory
- Principles of Magnetism. A. Abragam. Oxford Science Publication.
- Principles of pulse electron paramagnetic resonance. A. Schweiger, G. Jeschke.Oxford University Press.
-Electron Spin Resonance: a comprehensive treatise on experimental Techniques. C. P. Poole. Courier Dover Publications, 1996.
-EPR Spectroscopy. Applications in Chemistry and Biology. Topics in Current Chemistry 321. Eds. M. Drescher, G. Jeschke. Springer
-ESR Spectroscopy in membrane Biophysics. Eds. M.A. Hemminga,L.J. Berliner. Springer.