PUBLIKATIONEN

2024

Joseph B. Protein-protein interaction and conformational change in the alpha-helical membrane transporter BtuCD-F in the native cellular envelope.ChemBioChem https://doi.org/10.1002/cbic.202400858
Dajka M, Rath T, Morgner N and Joseph B. Dynamic basis of lipopolysaccharide export by LptB₂FGC. eLife https://doi.org/10.7554/eLife.99338.3
Gopinath A, Rath T, Morgner N, Joseph B. Lateral gating mechanism and plasticity of the BAM complex in micelles and E. coli. PNAS Nexus, 3, pgae019, https://doi.org/10.1093/pnasnexus/pgae019
Ketter S, Gopinath A, Joseph B. Conformational heterogeneity of β-barrel membrane proteins observed in situ using orthogonal spin labels and pulsed ESR spectroscopy. Methods. Mol. Biol., 2778:237-257, 10.1007/978-1-0716-3734-0_15

Wiedemann C, WhittakerJJ, Pérez Carrillo VH, Goretzki B, Dajka M, Tebbe F, Harder J.-M, Krajczy PR, Joseph B, Hausch F, Guskov A, Hellmich UA. Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding. Int. J. Biol. Macromol., 252, 126366, https://doi.org/10.1016/j.ijbiomac.2023.126366
Rudolph M, Tampé R, Joseph B. Time-resolved Mn2+–NO and NO–NO distance measurements reveal that catalytic asymmetry regulates alternating access in an ABC transporter. Angew. Chem. Int. Ed., 62, e2023070, https://doi.org/10.1002/anie.202307091
Ketter S, Joseph B. Gd(III) - trityl - nitroxide triple labelling and distance measurements in the heterooligomeric cobalamin transport complex in the native lipid bilayers. J. Am. Chem. Soc.,145, 2, 960–966, https://doi.org/10.1021/jacs.2c10080
Tait C and Joseph B. Editorial, 'Special Issue: Frontiers of EPR'. J. Magn. Reson., 356, 107563, https://doi.org/10.1016/j.jmr.2023.107563 (non-peer reviewed)

Ketter S, Dajka M, Rogozhnikova O, Dobrynin S, Tormyshev VM, Bhagryanskaya EG, Joseph B. In situ distance measurements in a membrane transporter using maleimide functionalized orthogonal spin labels and 5-pulse electron-electron double resonance spectroscopy. J. Magn. Reson. Open, 10, 100041, https://doi.org/10.1016/j.jmro.2022.100041
Gopinath A, Joseph B. Conformational flexibility of the protein insertase BamA in the native asymmetric bilayer elucidated with ESR spectroscopy. Angew. Chem. Int. Ed., 61, e202113448, https://doi.org/10.1002/anie.202113448

Kugele A, Ketter S, Silkenath B, Wittmann V, Joseph B,* Drescher M*. In situ EPR Spectroscopy of a Bacterial Membrane Transporter using an Expanded Genetic Code. ChemComm 57, 12980, https://doi.org/10.1039/D1CC04612H
Ketter S, Gopinath A, Rogozhnikova O, Trukhin D, Tormyshev VM, Bhagryanskaya EG, Joseph B. In situ labeling and distance measurements of membrane proteins in E. coli using Finland and OX063 trityl labels. Chem. Eur. J., 27, 2299-2304, https://doi.org/10.1002/chem.202004606

Barth K, Rudolph M, Diederichs T, Prisner TF, Tampé R, Joseph B. Thermodynamic Basis for Conformational Coupling in an ATP-Binding Cassette Exporter. J. Phys. Chem. Lett., 11, 7946-7953, https://doi.org/10.1021/acs.jpclett.0c01876

Joseph B*, Jaumann EA, Sikora A, Barth K, Prisner TF, Cafiso DS. In situ observation of conformational dynamics and protein-ligand/substrate interaction in outer membrane proteins with DEER/PELDOR spectroscopy. Nat. Protoc., 14, 2344-2369, https://doi.org/10.1038/s41596-019-0182-2
Yung-Ning Chang, Eva A. Jaumann, Katrin Reichel, Julia Hartmann, Dominik Oliver, Gerhard Hummer*, Benesh Joseph*, Eric R. Geertsma*. Structural basis for functional interactions in dimers of SLC26 transporters. Nat. Commun.,10, 2032, https://doi.org/10.1038/s41467-019-10001-w
Bock C, Löhr F, Tumulka F, Reichel K, Würz J, Hummer G, Schäfer L, Tampé R, Joseph B, Bernhard F, Dötsch V, Abele R. Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL. Sci. Rep., 8(1), 15662, https://doi.org/10.1038/s41598-018-33841-w

Barth K, Hank S, Spindler PE, Prisner TF, Tampé R, Joseph B. Conformational Coupling and trans-Inhibition in the Human Antigen Transporter Ortholog TmrAB Resolved with Dipolar EPR Spectroscopy. J. Am. Chem. Soc., 140, 4527–4533, https://doi.org/10.1021/jacs.7b12409

Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM,Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, StroudRM, Pos KM, and Tampé R. Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP. Proc. Natl. Acad. Sci. USA., 114, E438-447. https://doi.org/10.1073/pnas.1620009114

Sikora A, Joseph B, Matson M, Staley JR, Cafiso DS. Allosteric Signaling Is Bidirectional in an Outer-Membrane Transport Protein. Biophys. J., 111,1908-18, https://doi.org/10.1016/j.bpj.2016.09.038
Joseph B*, Tormyshev VM, Rogozhnikova OYu, Akhmetzyanov D, Bagryanskaya EG, Prisner TF*. Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes using Trityl - Nitroxide PELDOR. Angew. Chem. Int. Ed., 55,11538-42, https://doi.org/10.1002/anie.201606335
Joseph B*, Sikora A, Cafiso DS*. Ligand induced conformational changes in a membrane transporter in E. coli cell’s observed with DEER/PELDOR. J. Am. Chem. Soc., 138, 1844-47, (highlighted in JACS Spotlights) https://doi.org/10.1021/jacs.5b13382

Joseph B, Sikora A, Bordignon E, Jeschke G, Cafiso DS, Prisner TF. Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy. Angew. Chem. Int. Ed., 54, 6196-6199, https://doi.org/10.1002/anie.201501086

Joseph B, Korkhov VM, Yulikov M, Jeschke G, Bordignon E. Conformational cycle of the vitamin B12 ABC importer in liposomes detected by double electron-electron resonance (DEER). J. Biol. Chem., 289, 3176-3185, https://doi.org/10.1074/jbc.M113.512178

Doll A, Bordignon E, Joseph B, Tschaggelar R, Jeschke G. Liquid state DNP for water accessibility measurements on spin-labeled membrane proteins at physiological temperatures. J. Magn. Reson., 222, 34-43, https://doi.org/10.1016/j.jmr.2012.06.003

Joseph B, Jeschke G, Goetz BA, Locher KP, Bordignon E. Transmembrane gate movements in the type II ATP-binding cassette (ABC) importer BtuCD-F during nucleotide cycle. J. Biol. Chem., 286, 41008-41017, https://doi.org/10.1074/jbc.M111.269472

Kajiyama S, Joseph B, Inoue F, Shimamura M, Fukusaki E, Tomizawa K, Kobayashi A (2008) Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation. J. Biosci. Bioeng., 106, 194-198, https://doi.org/10.1263/jbb.106.194