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Bühl E, Resler T, Lam R, Asido M, Bamberg E, Schlesinger R, Bamann C, Heberle J, Wachtveitl J.
Assessing the Role of R120 in the Gating of CrChR2 by Time-Resolved Spectroscopy from Femtoseconds to Seconds
J Am Chem Soc. 2023 Sep 29. doi: 10.1021/jacs.3c05399. Epub 2023 Sep 29

Baserga F, Storm J, Schlesinger R, Heberle J, Stripp ST
The catalytic reaction of cytochrome c oxidase probed by in situ gas titrations and FTIR difference spectroscopy
Biochim Biophys Acta Bioenerg. 2023 Nov 1;1864(4):149000. doi: 10.1016/j.bbabio.2023.149000. Epub 2023 Jul 27
PMID: 37729557

Schubert L, Chen JL, Fritz T, Marxer F, Langner P, Hoffmann K, Gamiz-Hernandez AP, Kaila VRI, Schlesinger R, Heberle J.
Proton Release Reactions in the Inward H+ Pump NsXeR
J Phys Chem B. 2023 Sep 20. doi: 10.1021/acs.jpcb.3c04100. Online ahead of print.
PMID: 37729557

Walter M, Schubert L, Heberle J, Schlesinger R, Losi A
Time-resolved photoacoustics of channelrhodopsins: early energetics and light-driven volume changes
Photochem Photobiol Sci. 22, 477–486 (2023). doi: 10.1007/s43630-022-00327-8

Crea F, Vorkas A, Redlich A, Cruz R, Shi C, Trauner D, Lange A, Schlesinger R, Heberle J
Photoactivation of a Mechanosensitive Channel
Front. Mol. Biosci., 28 June 2022 | https://doi.org/10.3389/fmolb.2022.905306

Ataka K, Baumann A, Chen JL, Redlich A, Heberle J, Schlesinger R
Monitoring the progression of cell-free expression of microbial rhodopsins by Surface Enhanced IR spectroscopy: Resolving a branch point for successful/unsuccessful folding
Front Mol Biosci. 2022 July 14;9:929285.   doi: 10.3389/fmolb.2022.929285

Baserga F, Vorkas A, Crea F, Schubert L, Chen JL, Redlich A, La Greca M, Storm J, Oldemeyer S, Hoffmann K, Schlesinger R, Heberle J
Membrane Protein Activity Induces Specific Molecular Changes in Nanodiscs Monitored by FTIR Difference Spectroscopy
Front Mol Biosci. 2022 Jun 13;9:915328. doi: 10.3389/fmolb.2022.915328

La Greca M,  Chen J-L, Schubert L, Kozuch J, Berneiser T,  Terpitz U,  Heberle J, Schlesinger R
The Photoreaction of the Proton-Pumping Rhodopsin 1 From the Maize Pathogenic Basidiomycete Ustilago maydis
Front Mol. Biosci. 2022, doi.org/10.3389/fmolb.2022.826990

Yaguchi M, Jia X, Schlesinger R, Jiang X, Ataka K, Heberle J
Near-Infrared Activation of Sensory Rhodopsin II Mediated by NIR-to-Blue Upconversion Nanoparticles
Front. Mol. Biosci., 2022,   https://doi.org/10.3389/fmolb.2021.782688

Walter M, Schlesinger R
Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations
Methods Mol Biol. 2021;2191:29-48. doi: 10.1007/978-1-0716-0830-2_3. PMID: 3286573

Stripp ST, Oltmanns J, Müller CS, Ehrenberg D, Schlesinger R, Heberle J, Adrian L, Schünemann V, Pierik AJ, Soboh B.
Electron inventory of the iron-sulfur scaffold complex HypCD essential in [NiFe]-hydrogenase cofactor assembly
Biochem J (2021) 478 (17): 3281–3295. doi: 10.1042/BCJ20210224

Hugentobler, K. G., Heinrich, D., Berg, J., Heberle, J., Brzezinski, P., Schlesinger, R. and Block, S.
Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome c Oxidase
Int. J. Mol. Sci., 21(19), 6981 (2020); https://doi.org/10.3390/ijms21196981

Stauffer, M., Hirschi, S., Ucurum, Z., Harder, D., Schlesinger, R. and Fotiadis, D.
Engineering and Production of the Light-Driven Proton Pump Bacteriorhodopsin in 2D Crystals for Basic Research and Applied Technologies
Methods Protoc. 2020, 3(3), 51; https://doi.org/10.3390/mps3030051 - 22 Jul 2020

Kruse F., Nguyen A.D., Dragelj J., Schlesinger R., Heberle J., Mroginski M.A., Weidinger I.M.
Characterisation of the Cyanate Inhibited State of Cytochrome c Oxidase
Sci Rep. 2020 Mar 2;10(1):3863. doi: 10.1038/s41598-020-60801-0.

Stensitzki, T., Adam, S., Schlesinger, R., Schapiro, I., and Heyne, K.

Ultrafast Backbone Protonation in Channelrhodopsin-1 Captured by Polarization Resolved Fs Vis-pump—IR-Probe Spectroscopy and Computational Methods

Molecules 2020, 25(4), 848; https://doi.org/10.3390/molecules25040848 (registering DOI) - 14 Feb 2020

Ehrenberg, D., Krause, N., Saita, M., Bamann, C., Kar, R.K., Hoffmann, K.,  Heinrich, D., Schapiro, I., Heberle, J., and Schlesinger, R.
Atomistic insight into the role of threonine 127 in the functional mechanism of channelrhodopsin-2
Appl. Sci. 2019, 9(22), 4905; https://doi.org/10.3390/app922490

Nass Kovacs, G., Colletier, J.-P. , Grünbein, M. L., Yang, Y., Stensitzki, T. , Batyuk, A., Carbajo, S., Doak, R. B., Ehrenberg, D., Foucar, L., Gasper, R., Gorel, A.,  Hilpert, M., Kloos, M., Koglin, J. E., Reinstein, J., Roome, C. M., Schlesinger, R., Seaberg, M., Shoeman, R. L.,  Stricker, M., Boutet, S., Haacke, S.,  Heberle, J., Heyne, K., Domratcheva, T., Barends, T. R. M., and Schlichting, I.
Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
Nat. Commun. 10(1): 3177 (2019)

Saita, M, Pranga-Sellnau F, Resler T, Schlesinger R, Heberle J, Lórenz-Fonfría VA
Photoexcitation of the P4480 state induces a secondary photocycle that potentially desensitizes channelrhodopsin-2.
J Am Chem Soc. 140, 9899-9903 (2018); doi: 10.1021/jacs.8b03931

Pfitzner, E., Seki, H., Schlesinger, R., Ataka, K., Heberle, J.
Disc Antenna Enhanced Infrared Spectroscopy: From Self-Assembled Monolayers to Membrane Proteins
ACS Sens. 3, 5, 984-991 (2018), doi: 10.1021/acssensors.8b00139

Lorenz-Fonfria VA, Saita M, Lazarova T, Schlesinger R, Heberle J.
pH-sensitive vibrational probe reveals a cytoplasmic protonated cluster in bacteriorhodopsin.

Proc Natl Acad Sci U S A. 2017 Dec 4. pii: 201707993. doi: 10.1073/pnas.1707993114.

Schlesinger, R., Cousin, A., Granzin, J., Battra-Safferlind, R.
Expression and purification of arrestin in yeast Saccharomyces cerevisiae
Chapter in Methods in cell biology · January 2017 DOI: 10.1016/bs.mcb.2017.07.003

Schlesinger R.
Do It Fast: Immediate Functional Testing of Membrane Pumps Expressed into Nanodiscs
Biophys J. 2017 Aug 31. pii: S0006-3495(17)30912-8. doi: 10.1016/j.bpj.2017.08.020. [Epub ahead of print]

Harris NJ, Reading E, Ataka K, Grzegorzewski L, Charalambous K, Liu X, Schlesinger R, Heberle J, Booth PJ.
Structure formation during translocon-unassisted co-translational membrane protein folding
Sci Rep. 2017 Aug 14;7(1):8021. doi: 10.1038/s41598-017-08522-9

Orekhov P, Bothe A, Steinhoff HJ, Shaitan KV, Raunser S, Fotiadis D, Schlesinger R, Klare JP, Engelhard M.
Sensory Rhodopsin I and Sensory Rhodopsin II Form Trimers of Dimers in Complex with their Cognate Transducers
Photochem Photobiol. 2017 May;93(3):796-804. doi: 10.1111/php.12763. PMID: 28500714

Kovácsová, G., Grünbein, M.L., Kloos, M., Barends, T.R.M., Schlesinger, R., Heberle, J., Kabsch, W., Shoeman, R.L., Doaka, R.B., and Schlichting, I.
Viscous hydrophilic injection matrices for serial crystallography
IUCrJ (2017) 4, doi:10.1107/S2052252517005140

Sezer, M., Woelke, A. L., Knapp, E. W., Schlesinger, R., Mroginski, M. A. & Weidinger, I. M. (2016)
Redox induced protonation of heme propionates in cytochrome c oxidase: Insights from surface enhanced resonance Raman spectroscopy and QM/MM calculations.
Biochim Biophys Acta. 2017 Feb; 1858(2): 103-108. doi: 10.1016/j.bbabio.2016.10.009  Epub 2016 Oct 31.

Schreiner, M., Schlesinger, R., Heberle, J. & Niemann, H.H.
Crystal structure of Halobacterium salinarum halorhodopsin with a partially depopulated primary chloride-binding site
Acta Cryst. (2016). F72, 692-699, doi:10.1107/S2053230X16012796

Volz P, Krause N, Balke J, Schneider C, Walter M, Schneider F, Schlesinger R, Alexiev U. (2016)
Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2.
J Biol Chem. 2016 Aug 12;291(33):17382-93. doi: 10.1074/jbc.M115.711200. Epub 2016 Jun 6.

Stensitzki T, Yang Y, Muders V, Schlesinger R, Heberle J, Heyne K. (2016)
Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization
Struct. Dyn. 3, 043208.

Schnedermann C, Muders V, Ehrenberg D, Schlesinger R, Kukura P, Heberle J. (2016)
Vibronic Dynamics of the Ultrafast all-trans to 13-cis Photoisomerization of Retinal in Channelrhodopsin-1.
J Am Chem Soc. 138, 4757-62.

Baumann A, Kerruth S, Fitter J, Büldt G, Heberle J, Schlesinger R, Ataka K. (2016)
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression.
PLoS One. 11, e0151051.

Granzin, J., Stadler, A., Cousin, A., Schlesinger, R. & Batra-Safferling, R. (2015)
Structural evidence for the role of polar core residue Arg175 in arrestin activation,
Sci Rep.
5, 15808.

Lorenz-Fonfria, V. A., Bamann, C., Resler, T., Schlesinger, R., Bamberg, E. & Heberle, J. (2015)
Temporal evolution of helix hydration in a light-gated ion channel correlates with ion conductance,
Proceedings of the National Academy of Sciences of the United States of America.
112, E5796-804.

Stensitzki, T., Muders, V., Schlesinger, R., Heberle, J. & Heyne, K. (2015)
The primary photoreaction of channelrhodopsin-1: wavelength dependent photoreactions induced by ground-state heterogeneity,
Front Mol Biosci.
2, 41.

Sezer, M., Kielb, P., Kuhlmann, U., Mohrmann, H., Schulz, C., Heinrich, D., Schlesinger, R., Heberle, J. & Weidinger, I. M. (2015)
Surface Enhanced Resonance Raman Spectroscopy Reveals Potential Induced Redox and Conformational Changes of Cytochrome c Oxidase on Electrodes,
J Phys Chem B.
119, 9586-91.

Schreiner, M., Schlesinger, R., Heberle, J. & Niemann, H. H. (2015)
Structure of Halorhodopsin from Halobacterium salinarum in a new crystal form that imposes little restraint on the E-F loop,
J Struct Biol. 190, 373-8.

Lorenz-Fonfria, V. A., Schultz, B. J., Resler, T., Schlesinger, R., Bamann, C., Bamberg, E. & Heberle, J. (2015)
Pre-gating conformational changes in the ChETA variant of channelrhodopsin-2 monitored by nanosecond IR spectroscopy,
Journal of the American Chemical Society. 137, 1850-61.

Lorenz-Fonfria, V. A., Muders, V., Schlesinger, R. & Heberle, J. (2014)
Changes in the hydrogen-bonding strength of internal water molecules and cysteine residues in the conductive state of channelrhodopsin-1,
J Chem Phys.
141, 22D507.

Muders, V., Kerruth, S., Lorenz-Fonfria, V. A., Bamann, C., Heberle, J. & Schlesinger, R. (2014)
Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1,
FEBS letters.
588, 2301-6.

Ter Beek J, Krause N, Reimann J, Lachmann P, Aumldelroth P. (2013)

The NO reductase from Paracoccus denitrificans uses a single specific proton pathway,

J Biol Chem
. 288, 30626–30635

Krause, N., Engelhard, C., Heberle, J., Schlesinger, R. & Bittl, R. (2013)
Structural differences between the closed and open states of channelrhodopsin-2 as observed by EPR spectroscopy,
FEBS letters.
587, 3309-13.

Lorenz-Fonfria, V. A., Resler, T., Krause, N., Nack, M., Gossing, M., Fischer von Mollard, G., Bamann, C., Bamberg, E., Schlesinger, R. & Heberle, J. (2013)
Transient protonation changes in channelrhodopsin-2 and their relevance to channel gating,
Proceedings of the National Academy of Sciences of the United States of America.
110, E1273-81.

Salomonsson L, Reimann J, Tosha T, Krause N, Gonska N, Shiro Y, Adelroth P. (2012)
Proton transfer in the quinol-dependent nitric oxide reductase from Geobacillus stearothermophilus during reduction of oxygen.
Biochim Biophys Acta
. 1817, 1914-20.

Kubicek, J., Schlesinger, R., Baeken, C., Buldt, G., Schafer, F. & Labahn, J. (2012)
Controlled in meso phase crystallization--a method for the structural investigation of membrane proteins,
PLoS One.
7, e35458.

Nack, M., Radu, I., Schultz, B. J., Resler, T., Schlesinger, R., Bondar, A. N., del Val, C., Abbruzzetti, S., Viappiani, C., Bamann, C., Bamberg, E. & Heberle, J. (2012)
Kinetics of proton release and uptake by channelrhodopsin-2,
FEBS letters. 586, 1344-8.

Granzin, J., Cousin, A., Weirauch, M., Schlesinger, R., Buldt, G. & Batra-Safferling, R. (2012)
Crystal structure of p44, a constitutively active splice variant of visual arrestin,
Journal of molecular biology.
416, 611-8.

Erhardt H, Steimle S, Muders V, Pohl T, Walter J, Friedrich T. (2012)
Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli.
Biochim Biophys Acta
. 1817, 863-71.

Kirchberg, K., Kim, T. Y., Moller, M., Skegro, D., Dasara Raju, G., Granzin, J., Buldt, G., Schlesinger, R. & Alexiev, U. (2011)
Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process,
Proceedings of the National Academy of Sciences of the United States of America.
108, 18690-5.

Katranidis, A., Grange, W., Schlesinger, R., Choli-Papadopoulou, T., Bruggemann, D., Hegner, M. & Buldt, G. (2011)
Force measurements of the disruption of the nascent polypeptide chain from the ribosome by optical tweezers,
FEBS letters. 585, 1859-63.

Fitter, J., Katranidis, A., Rosenkranz, T., Atta, D., Schlesinger R., and Büldt G. (2011)
Single molecule fluorescence spectroscopy: a tool for protein studies approaching cellular environmental conditions,
Soft Matter
, Advance Article DOI: 10.1039/C0SM00538J, Emerging Area

Rosenkranz, T., Schlesinger, R., Gabba, M. & Fitter, J. (2011)
Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRET,
12, 704-10.

Radu, I., Budyak, I. L., Hoomann, T., Kim, Y. J., Engelhard, M., Labahn, J., Buldt, G., Heberle, J. & Schlesinger, R. (2010)
Signal relay from sensory rhodopsin I to the cognate transducer HtrI: assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53,
Biophys Chem.
150, 23-8.

Katranidis, A., Atta, D., Schlesinger, R., Nierhaus, K. H., Choli-Papadopoulou, T., Gregor, I., Gerrits, M., Buldt, G. & Fitter, J. (2009)
Fast biosynthesis of GFP molecules: a single-molecule fluorescence study,
Angew Chem Int Ed Engl.
48, 1758-61.

Budyak, I. L., Mironova, O. S., Yanamala, N., Manoharan, V., Buldt, G., Schlesinger, R. & Klein-Seetharaman, J. (2008)
Flexibility of the cytoplasmic domain of the phototaxis transducer II from Natronomonas pharaonis,
J Biophys.
2008, 267912.

Jiang, X., Zaitseva, E., Schmidt, M., Siebert, F., Engelhard, M., Schlesinger, R., Ataka, K., Vogel, R. & Heberle, J. (2008)
Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy,
Proceedings of the National Academy of Sciences of the United States of America.
105, 12113-7.

Mironova, O. S., Budyak, I. L., Buldt, G., Schlesinger, R. & Heberle, J. (2007)
FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI,
46, 9399-405.

Immeln, D., Schlesinger, R., Heberle, J. & Kottke, T. (2007)
Blue light induces radical formation and autophosphorylation in the light-sensitive domain of Chlamydomonas cryptochrome,
The Journal of biological chemistry.
282, 21720-8.

Mennes, N., Klare, J. P., Chizhov, I., Seidel, R., Schlesinger, R. & Engelhard, M. (2007)
Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli,
FEBS letters.
581, 1487-94.

Skegro, D., Pulvermüller, A., Krafft, B., Granzin, J., Hofmann, K. P., Buldt, G. & Schlesinger, R. (2007)
N-terminal and C-terminal domains of arrestin both contribute in binding to rhodopsin,
Photochem Photobiol.
83, 385-92.

Budyak, I. L., Pipich, V., Mironova, O. S., Schlesinger, R., Zaccai, G. & Klein-Seetharaman, J. (2006)
Shape and oligomerization state of the cytoplasmic domain of the phototaxis transducer II from Natronobacterium pharaonis,
Proceedings of the National Academy of Sciences of the United States of America.
103, 15428-33.

Schenkl, S., van Mourik, F., Friedman, N., Sheves, M., Schlesinger, R., Haacke, S. & Chergui, M. (2006)
Insights into excited-state and isomerization dynamics of bacteriorhodopsin from ultrafast transient UV absorption,
Proceedings of the National Academy of Sciences of the United States of America.
103, 4101-6.

Mironova, O. S., Efremov, R. G., Person, B., Heberle, J., Budyak, I. L., Buldt, G. & Schlesinger, R. (2005)
Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli,
FEBS letters. 579, 3147-51.

Hwang, J. Y., Schlesinger, R. & Koch, K. W. (2004)
Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation,
Eur J Biochem.
271, 3785-93.

Gordeliy VI, Schlesinger R, Efremov R, Büldt G, Heberle J. (2003)
Crystallization in lipidic cubic phases: a case study with bacteriorhodopsin
Methods Mol Biol. 228, 305-16

Choe, H. W., Jeong, D. G., Park, J. H., Schlesinger, R., Labahn, J., Hofmann, K. P. & Buldt, G. (2003)
Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli: expression, crystallization and cryoconditions,
Acta Crystallogr D Biol Crystallogr.
59, 350-2.

Gordeliy, V. I., Labahn, J., Moukhametzianov, R., Efremov, R., Granzin, J., Schlesinger, R., Buldt, G., Savopol, T., Scheidig, A. J., Klare, J. P. & Engelhard, M. (2002)
Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex,
419, 484-7.

Hwang, J. Y., Schlesinger, R. & Koch, K. W. (2001)
Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1,
FEBS letters.
508, 355-9.

Zscherp, C., Schlesinger, R. & Heberle, J. (2001)
Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin,
Biochem Biophys Res Commun.
283, 57-63.

Sass, H. J., Büldt, G., Gessenich, R., Hehn, D., Neff, D., Schlesinger, R., Berendzen, J. & Ormos, P. (2000)
Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin,
406, 649-53.

Büldt G, Schlesinger R, Pebay-Peroula E, Sass HJ (2000)
Structure and Dynamics of Biological Membranes.
Oxford University Press, „Structure and Dynamics of Biomolecules“ Eds. E. Franchon et al., 251-271

Bungert, S., Krafft, B., Schlesinger, R. & Friedrich, T. (1999)
One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography,
FEBS letters.
460, 207-11.

Zscherp, C., Schlesinger, R., Tittor, J., Oesterhelt, D. & Heberle, J. (1999)
In situ determination of transient pKa changes of internal amino acids of bacteriorhodopsin by using time-resolved attenuated total reflection Fourier-transform infrared spectroscopy,
Proceedings of the National Academy of Sciences of the United States of America.
96, 5498-503.

Büldt G, Heberle J, Schlesinger R, Sass HJ (1999)
Structure, dynamics and function of the proton pump bacteriorhodopsin
MPSA Book „Proteome ans Protein Analysis“, Springer-Verlag, 225-240

Schlesinger, R., Kahmann, R. & Kämper, J. (1997)
The homeodomains of the heterodimeric bE and bW proteins of Ustilago maydis are both critical for function,
Mol Gen Genet.
254, 514-9.

Bölker M, Dahl M, Schlesinger R, Bergemann J, Gillissen B, Schauwecker F, Urban M, Schroeer B, Kahmann R (1991)
Mating type genes of Ustilago maydis.
Molecular biology of filamentous fungi: Proceedings of the EMBO Workshop, Berlin.
Eds. U Stahl, P Tudzynski

Schulz, B., Banuett, F., Dahl, M., Schlesinger, R., Schafer, W., Martin, T., Herskowitz, I. & Kahmann, R. (1990)
The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif,
Cell. 60, 295-306.

Kreft, J., Funke, D., Schlesinger, R., Lottspeich, F. & Goebel, W. (1989)
Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii,
Acta Microbiol Hung.
36, 189-92.