Molecular Bioenergetics in Oxygenic Photosynthesis
Obligatory preview (distribution of seminar talks, materials needed):
Tuesday, the 19.07.2016, 03:00-04:00 p.m.; R 1.1.16
Course (see time table above):
19.09.-23.09.2016, 9:00 a.m.-18:00 p.m.
Written examination (mixture of lecture, seminar and practical topics):
Monday, the 26.09. 2016, 10:15-11:45 a.m.; R 1.1.53
Lab essentials: Lab coat (available) & sturdy shoes; hair tie (for fixing long hair); gloves, googles and filter masks (provided); warm jackets, trousers and shoes (especially for Tuesday)
Writing things: pencil, permanent marker (available), ruler, calculator (optional), Laptop (optional), script (provided)
Please, do not forget to bring sufficient drinks and snacks for small breaks with you. It is a long day!
Lecture, lab training:
English: Photosynthesis : molecular, physiological and environmental processes by David W. Lawlor 2. ed., Harlow u.a. : Longman u.a., 1993
Deutsch: Photosynthese : Stoffwechsel, Kontrolle, Physiologie bei David W. Lawlor; Donat-Peter Häder Stuttgart u.a. : Thieme, 1990
English: The tools of biochemistry by Terrance G. Cooper; New York [u.a.] : Wiley, 1977
Deutsch: Biochemische Arbeitsmethoden. The tools of biochemistry bei Terrance G. Cooper; Reinhard Neumeier [Übers.] Berlin [u.a.] : de Gruyter, 1981
The golden thread of the practical
The interdisciplinary module “Bioenergetics in Oxygenic Photosynthesis” highlights basic processes and methods in classical and current Photosynthesis research. The course guide you theoretically (lectures) and practically (lab) through the four phases of energy storage in photosynthesis: (1) light absorption and energy delivery by antenna systems, (2) primary electron transfer reactions, (3) energy stabilization by secondary processes (ATP, NADPH biosynthesis), and (4) synthesis and export of stable products (glycogen, starch). For this purpose, you are introduced to cellular light adaption processes such as Chromatic Adaptation and respective pigment analysis by both thin layer chromatography and spectroscopy (day 1). At the following day (day 2), you will isolate yourself thylakoid membranes (in the green dark light) from different thermophilic mutant strains varying in the composition of photosystem II. These kind of biological samples are analyzed by polyacrylamide-electrophoresis and Western Blot techniques (day 3), by prompt and delayed fluorescence (day 4) as well as by oxygen polarography (day 5). We will assist in data analysis and finally discuss your results in the context of mechanistic in photosynthetic reactions. Moreover, you present your coworkers theoretically (seminar presentation or demonstration) and get trained practically in basic molecular, biochemical and biophysical techniques. The course gives you an exciting opportunity to become familiar with the field of general and applied photosynthesis and to improve your skills in managing lab basics and running experiments in a comprehensive and reproducible manner.