13.04. 10 - 12 am Introduction to the practical (administration) as part of the lecture. 19.04. 10 - 12 am MANDATORY introduction to lab and LASER safety. Also, DEADLINE for attendance of practical.
10 ECTS only together with lecture 20239! This module will present and substantiate biophysical methods and concepts. Selected methods like spectroscopy and diffraction and their application to proteins, nucleic acids and biomembranes are of particular relevance. The lecture series will cover a selection of the following methods: absorption spectroscopy in the UV, visible and IR region; fluorescence spectroscopy, time-resolved approaches; spectroscopy with linear and circular polarized light; vibrational spectroscopy: Fourier-transform infrared (FTIR), resonance Raman, surfance-enhanced Raman and IR; diffraction with X-rays, Neutrons and electrons; crystallization and protein crystallography; nuclear magnetic resonance (NMR); light scattering; single molecule spectroscopy; optical tweezer; atomic force microscopy; theoretical methods: MD simulations, Poisson-Boltzmann, QM/MM
Tuszynski & Kurzynski: Introduction to Molecular Biophysics. Cantor & Schimmel: Biophysical Chemistry. Walla: Modern Biophysical Chemistry. Brandén & Tooze: Introduction to Protein Structure. Winter & Noll: Methoden der Biophysikalischen Chemie. Gennis: Biomembranes