laborbilder584x102

Publications

Sezer, M., Woelke, A. L., Knapp, E. W., Schlesinger, R., Mroginski, M. A. & Weidinger, I. M. (2016)
Redox induced protonation of heme propionates in cytochrome c oxidase: Insights from surface enhanced resonance Raman spectroscopy and QM/MM calculations.
Biochim Biophys Acta. Oct 31. pii: S0005-2728(16)30652-1. doi: 10.1016/j.bbabio.2016.10.009


Schreiner, M., Schlesinger, R., Heberle, J. & Niemann, H.H. (2016)
Crystal structure of Halobacterium salinarum halorhodopsin with a partially depopulated primary chloride-binding site
Acta Cryst.  F72 , doi:10.1107/S2053230X16012796


Volz P, Krause N, Balke J, Schneider C, Walter M, Schneider F, Schlesinger R, Alexiev U.
Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2.
J Biol Chem. 2016 Aug 12;291(33):17382-93. doi: 10.1074/jbc.M115.711200. Epub 2016 Jun 6.


Stensitzki T, Yang Y, Muders V, Schlesinger R, Heberle J, Heyne K. (2016)
Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization
Struct. Dyn. 3, 043208.

Schnedermann C, Muders V, Ehrenberg D, Schlesinger R, Kukura P, Heberle J. (2016)
Vibronic Dynamics of the Ultrafast all-trans to 13-cis Photoisomerization of Retinal in Channelrhodopsin-1.
J Am Chem Soc. 138, 4757-62.


Baumann A, Kerruth S, Fitter J, Büldt G, Heberle J, Schlesinger R, Ataka K. (2016)
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression.
PLoS One. 11, e0151051.


Granzin, J., Stadler, A., Cousin, A., Schlesinger, R. & Batra-Safferling, R. (2015)
Structural evidence for the role of polar core residue Arg175 in arrestin activation,
Sci Rep.
5, 15808.


Lorenz-Fonfria, V. A., Bamann, C., Resler, T., Schlesinger, R., Bamberg, E. & Heberle, J. (2015)
Temporal evolution of helix hydration in a light-gated ion channel correlates with ion conductance,
Proceedings of the National Academy of Sciences of the United States of America.
112, E5796-804.


Stensitzki, T., Muders, V., Schlesinger, R., Heberle, J. & Heyne, K. (2015)
The primary photoreaction of channelrhodopsin-1: wavelength dependent photoreactions induced by ground-state heterogeneity,
Front Mol Biosci.
2, 41.


Sezer, M., Kielb, P., Kuhlmann, U., Mohrmann, H., Schulz, C., Heinrich, D., Schlesinger, R., Heberle, J. & Weidinger, I. M. (2015)
Surface Enhanced Resonance Raman Spectroscopy Reveals Potential Induced Redox and Conformational Changes of Cytochrome c Oxidase on Electrodes,
J Phys Chem B.
119, 9586-91.


Schreiner, M., Schlesinger, R., Heberle, J. & Niemann, H. H. (2015)
Structure of Halorhodopsin from Halobacterium salinarum in a new crystal form that imposes little restraint on the E-F loop,
J Struct Biol. 190, 373-8.


Lorenz-Fonfria, V. A., Schultz, B. J., Resler, T., Schlesinger, R., Bamann, C., Bamberg, E. & Heberle, J. (2015)
Pre-gating conformational changes in the ChETA variant of channelrhodopsin-2 monitored by nanosecond IR spectroscopy,
Journal of the American Chemical Society. 137, 1850-61.


Lorenz-Fonfria, V. A., Muders, V., Schlesinger, R. & Heberle, J. (2014)
Changes in the hydrogen-bonding strength of internal water molecules and cysteine residues in the conductive state of channelrhodopsin-1,
J Chem Phys.
141, 22D507.


Muders, V., Kerruth, S., Lorenz-Fonfria, V. A., Bamann, C., Heberle, J. & Schlesinger, R. (2014)
Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1,
FEBS letters.
588, 2301-6.


Ter Beek J, Krause N, Reimann J, Lachmann P, Aumldelroth P. (2013)

The NO reductase from Paracoccus denitrificans uses a single specific proton pathway,

J Biol Chem
. 288, 30626–30635


Krause, N., Engelhard, C., Heberle, J., Schlesinger, R. & Bittl, R. (2013)
Structural differences between the closed and open states of channelrhodopsin-2 as observed by EPR spectroscopy,
FEBS letters.
587, 3309-13.


Lorenz-Fonfria, V. A., Resler, T., Krause, N., Nack, M., Gossing, M., Fischer von Mollard, G., Bamann, C., Bamberg, E., Schlesinger, R. & Heberle, J. (2013)
Transient protonation changes in channelrhodopsin-2 and their relevance to channel gating,
Proceedings of the National Academy of Sciences of the United States of America.
110, E1273-81.


Salomonsson L, Reimann J, Tosha T, Krause N, Gonska N, Shiro Y, Adelroth P. (2012)
Proton transfer in the quinol-dependent nitric oxide reductase from Geobacillus stearothermophilus during reduction of oxygen.
Biochim Biophys Acta
. 1817, 1914-20.


Kubicek, J., Schlesinger, R., Baeken, C., Buldt, G., Schafer, F. & Labahn, J. (2012)
Controlled in meso phase crystallization--a method for the structural investigation of membrane proteins,
PLoS One.
7, e35458.


Nack, M., Radu, I., Schultz, B. J., Resler, T., Schlesinger, R., Bondar, A. N., del Val, C., Abbruzzetti, S., Viappiani, C., Bamann, C., Bamberg, E. & Heberle, J. (2012)
Kinetics of proton release and uptake by channelrhodopsin-2,
FEBS letters. 586, 1344-8.


Granzin, J., Cousin, A., Weirauch, M., Schlesinger, R., Buldt, G. & Batra-Safferling, R. (2012)
Crystal structure of p44, a constitutively active splice variant of visual arrestin,
Journal of molecular biology.
416, 611-8.


Erhardt H, Steimle S, Muders V, Pohl T, Walter J, Friedrich T. (2012)
Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli.
Biochim Biophys Acta
. 1817, 863-71.


Kirchberg, K., Kim, T. Y., Moller, M., Skegro, D., Dasara Raju, G., Granzin, J., Buldt, G., Schlesinger, R. & Alexiev, U. (2011)
Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process,
Proceedings of the National Academy of Sciences of the United States of America.
108, 18690-5.


Katranidis, A., Grange, W., Schlesinger, R., Choli-Papadopoulou, T., Bruggemann, D., Hegner, M. & Buldt, G. (2011)
Force measurements of the disruption of the nascent polypeptide chain from the ribosome by optical tweezers,
FEBS letters. 585, 1859-63.


Fitter, J., Katranidis, A., Rosenkranz, T., Atta, D., Schlesinger R., and Büldt G. (2011)
Single molecule fluorescence spectroscopy: a tool for protein studies approaching cellular environmental conditions,
Soft Matter
, Advance Article DOI: 10.1039/C0SM00538J, Emerging Area


Rosenkranz, T., Schlesinger, R., Gabba, M. & Fitter, J. (2011)
Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRET,
Chemphyschem.
12, 704-10.


Radu, I., Budyak, I. L., Hoomann, T., Kim, Y. J., Engelhard, M., Labahn, J., Buldt, G., Heberle, J. & Schlesinger, R. (2010)
Signal relay from sensory rhodopsin I to the cognate transducer HtrI: assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53,
Biophys Chem.
150, 23-8.


Katranidis, A., Atta, D., Schlesinger, R., Nierhaus, K. H., Choli-Papadopoulou, T., Gregor, I., Gerrits, M., Buldt, G. & Fitter, J. (2009)
Fast biosynthesis of GFP molecules: a single-molecule fluorescence study,
Angew Chem Int Ed Engl.
48, 1758-61.


Budyak, I. L., Mironova, O. S., Yanamala, N., Manoharan, V., Buldt, G., Schlesinger, R. & Klein-Seetharaman, J. (2008)
Flexibility of the cytoplasmic domain of the phototaxis transducer II from Natronomonas pharaonis,
J Biophys.
2008, 267912.


Jiang, X., Zaitseva, E., Schmidt, M., Siebert, F., Engelhard, M., Schlesinger, R., Ataka, K., Vogel, R. & Heberle, J. (2008)
Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy,
Proceedings of the National Academy of Sciences of the United States of America.
105, 12113-7.


Mironova, O. S., Budyak, I. L., Buldt, G., Schlesinger, R. & Heberle, J. (2007)
FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI,
Biochemistry.
46, 9399-405.


Immeln, D., Schlesinger, R., Heberle, J. & Kottke, T. (2007)
Blue light induces radical formation and autophosphorylation in the light-sensitive domain of Chlamydomonas cryptochrome,
The Journal of biological chemistry.
282, 21720-8.


Mennes, N., Klare, J. P., Chizhov, I., Seidel, R., Schlesinger, R. & Engelhard, M. (2007)
Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli,
FEBS letters.
581, 1487-94.


Skegro, D., Pulvermüller, A., Krafft, B., Granzin, J., Hofmann, K. P., Buldt, G. & Schlesinger, R. (2007)
N-terminal and C-terminal domains of arrestin both contribute in binding to rhodopsin,
Photochem Photobiol.
83, 385-92.


Budyak, I. L., Pipich, V., Mironova, O. S., Schlesinger, R., Zaccai, G. & Klein-Seetharaman, J. (2006)
Shape and oligomerization state of the cytoplasmic domain of the phototaxis transducer II from Natronobacterium pharaonis,
Proceedings of the National Academy of Sciences of the United States of America.
103, 15428-33.


Schenkl, S., van Mourik, F., Friedman, N., Sheves, M., Schlesinger, R., Haacke, S. & Chergui, M. (2006)
Insights into excited-state and isomerization dynamics of bacteriorhodopsin from ultrafast transient UV absorption,
Proceedings of the National Academy of Sciences of the United States of America.
103, 4101-6.


Mironova, O. S., Efremov, R. G., Person, B., Heberle, J., Budyak, I. L., Buldt, G. & Schlesinger, R. (2005)
Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli,
FEBS letters. 579, 3147-51.


Hwang, J. Y., Schlesinger, R. & Koch, K. W. (2004)
Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation,
Eur J Biochem.
271, 3785-93.


Choe, H. W., Jeong, D. G., Park, J. H., Schlesinger, R., Labahn, J., Hofmann, K. P. & Buldt, G. (2003)
Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli: expression, crystallization and cryoconditions,
Acta Crystallogr D Biol Crystallogr.
59, 350-2.


Gordeliy, V. I., Labahn, J., Moukhametzianov, R., Efremov, R., Granzin, J., Schlesinger, R., Buldt, G., Savopol, T., Scheidig, A. J., Klare, J. P. & Engelhard, M. (2002)
Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex,
Nature.
419, 484-7.


Hwang, J. Y., Schlesinger, R. & Koch, K. W. (2001)
Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1,
FEBS letters.
508, 355-9.


Zscherp, C., Schlesinger, R. & Heberle, J. (2001)
Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin,
Biochem Biophys Res Commun.
283, 57-63.


Sass, H. J., Büldt, G., Gessenich, R., Hehn, D., Neff, D., Schlesinger, R., Berendzen, J. & Ormos, P. (2000)
Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin,
Nature.
406, 649-53.


Büldt G, Schlesinger R, Pebay-Peroula E, Sass HJ (2000)
Structure and Dynamics of Biological Membranes.
Oxford University Press, „Structure and Dynamics of Biomolecules“ Eds. E. Franchon et al., 251-271


Bungert, S., Krafft, B., Schlesinger, R. & Friedrich, T. (1999)
One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography,
FEBS letters.
460, 207-11.


Zscherp, C., Schlesinger, R., Tittor, J., Oesterhelt, D. & Heberle, J. (1999)
In situ determination of transient pKa changes of internal amino acids of bacteriorhodopsin by using time-resolved attenuated total reflection Fourier-transform infrared spectroscopy,
Proceedings of the National Academy of Sciences of the United States of America.
96, 5498-503.


Büldt G, Heberle J, Schlesinger R, Sass HJ (1999)
Structure, dynamics and function of the proton pump bacteriorhodopsin
MPSA Book „Proteome ans Protein Analysis“, Springer-Verlag, 225-240


Schlesinger, R., Kahmann, R. & Kämper, J. (1997)
The homeodomains of the heterodimeric bE and bW proteins of Ustilago maydis are both critical for function,
Mol Gen Genet.
254, 514-9.


Bölker M, Dahl M, Schlesinger R, Bergemann J, Gillissen B, Schauwecker F, Urban M, Schroeer B, Kahmann R (1991)
Mating type genes of Ustilago maydis.
Molecular biology of filamentous fungi: Proceedings of the EMBO Workshop, Berlin.
Eds. U Stahl, P Tudzynski


Schulz, B., Banuett, F., Dahl, M., Schlesinger, R., Schafer, W., Martin, T., Herskowitz, I. & Kahmann, R. (1990)
The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif,
Cell. 60, 295-306.


Kreft, J., Funke, D., Schlesinger, R., Lottspeich, F. & Goebel, W. (1989)
Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii,
Acta Microbiol Hung.
36, 189-92.