Laser in AG Alexiev

Projects

Bioenergetics

  • Proton transport across biological membranes
  • Function of cytochrome c oxidase

Structural Dynamics and Molecular Recognition

  • Activation and molecular recognition of rhodopsins:
    Investigation of loop dynamics using time-resolved fluorescence depolarization and role of proton uptake
  • Nanodynamics of MHC class I complexes and their role in autoimmune diseases
  • Protein-DNA interaction

 

Methods

  • ps- and fs- time-resolved fluorescence depolarisation
  • Pump-probe fluorescence spectroscopy
  • Surface enhanced 2-photon fluorescence spectroscopy
  • Time-resolved absorption and FTIR spectroscopy
  • Protein biochemistry
  • TIRF microscopy
  • FLIM / FCS

 

 

Fundings

  • SFB 1078 Project A2: Surface protonation and conformational dynamics in cytochrome c oxidase and photoreceptors
  • SFB 1112 Project B03: Characterization of transport and release properties of responsive nanocarriers by fluorescence  spectroscopy and localization microscopy

Former Projects

  • Sfb 449 TP A5: Collaboration with PD Dr. O.P. Ernst and Prof. K.P. Hofmann (rhodopsins), with Dr. B. Uchanska-Ziegler (MHC complexes)
  • VW-Stiftung: Nanodynamics of MHC/Peptide complexes and its dependence on MHC polymorphism (Collaborations with Dr. R. Böckmann, Prof. K. Rück-Braun, Prof. R. Sorrentino, Prof. A. Ziegler)

Collaborations

  • Molecular dynamics simulation of loop dynamics: Collaboration with Prof. H. Grubmüller
  • Cytochrome c Oxidase: Collaboration with Prof. H. Michel
  • Grating waveguide structures: Collaboration with Laser-Laboratorium Göttingen