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Publications

2024

  • Dajka M, Rath T, Morgner N and Joseph B. Dynamic basis of lipopolysaccharide export by LptB2FGC. Under review in eLife. bioRxiv - https://doi.org/10.1101/2024.05.20.594984
  • Gopinath A, Rath T, Morgner N, Joseph B. Lateral gating mechanism and plasticity of the BAM complex in micelles and E. coli. PNAS Nexus, 3, pgae019, https://doi.org/10.1093/pnasnexus/pgae019
  • Ketter S, Gopinath A, Joseph B. Conformational heterogeneity of β-barrel membrane proteins observed in situ using orthogonal spin labels and pulsed ESR spectroscopy. Methods. Mol. Biol.10.1007/978-1-0716-3734-0_15

2023

  • Wiedemann C, Whittaker JJ, Pérez Carrillo VH, Goretzki B, Dajka M, Tebbe F, Harder J.-M, Krajczy PR, Joseph B, Hausch F, Guskov A, Hellmich UA. Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding. Int. J. Biol. Macromol., 252, 126366, https://doi.org/10.1016/j.ijbiomac.2023.126366
  • Rudolph M, Tampé R, Joseph B. Time-resolved Mn2+–NO and NO–NO distance measurements reveal that catalytic asymmetry regulates alternating access in an ABC transporter. Angew. Chem. Int. Ed., 62, e2023070 https://doi.org/10.1002/anie.202307091
  • Ketter S, Joseph B. Gd(III) - trityl - nitroxide triple labelling and distance measurements in the heterooligomeric cobalamin transport complex in the native lipid bilayers. J. Am. Chem. Soc.,145, 2, 960–966, https://doi.org/10.1021/jacs.2c10080
  • Tait C and Joseph B. Editorial, 'Special Issue: Frontiers of EPR'. J. Magn. Reson., 356, 107563, https://doi.org/10.1016/j.jmr.2023.107563 (non-peer reviewed)

2022

  • Ketter S, Dajka M, Rogozhnikova O,  Dobrynin S, Tormyshev VM, Bhagryanskaya EG, Joseph B. In situ distance measurements in a membrane transporter using maleimide functionalized orthogonal spin labels and 5-pulse electron-electron double resonance spectroscopy.  J. Magn. Reson. Open, 10, 100041, https://doi.org/10.1016/j.jmro.2022.100041
  • Gopinath A, Joseph B. Conformational flexibility of the protein insertase BamA in the native asymmetric bilayer elucidated with ESR spectroscopy.  Angew. Chem. Int. Ed., 61, e202113448, https://doi.org/10.1002/anie.202113448

2021

  • Kugele A, Ketter S, Silkenath B, Wittmann V, Joseph B,* Drescher M*. In situ EPR Spectroscopy of a Bacterial Membrane Transporter using an Expanded Genetic Code.  ChemComm  57, 12980, https://doi.org/10.1039/D1CC04612H
  • Ketter S, Gopinath A, Rogozhnikova O, Trukhin D, Tormyshev VM, Bhagryanskaya EG, Joseph B. In situ labeling and distance measurements of membrane proteins in E. coli using Finland and OX063 trityl labels. Chem. Eur. J., 27, 2299-2304, https://doi.org/10.1002/chem.202004606

2020

  • Barth K, Rudolph M,  Diederichs T, Prisner TF, Tampé R*, Joseph B. Thermodynamic Basis for Conformational Coupling in an ATP-Binding Cassette Exporter. J. Phys. Chem. Lett., 11, 7946-7953, https://doi.org/10.1021/acs.jpclett.0c01876

2019

  • Joseph B*, Jaumann EA, Sikora A, Barth K, Prisner TF, Cafiso DS. In situ observation of conformational dynamics and protein-ligand/substrate interaction in outer membrane proteins with DEER/PELDOR spectroscopy. Nat. Protoc., 14, 2344-2369, https://doi.org/10.1038/s41596-019-0182-2
  • Yung-Ning Chang, Eva A. Jaumann, Katrin Reichel, Julia Hartmann, Dominik Oliver, Gerhard Hummer*, Benesh Joseph*, Eric R. Geertsma*. Structural basis for functional interactions in dimers of SLC26 transporters. Nat. Commun.,10, 2032, https://doi.org/10.1038/s41467-019-10001-w
  • Bock C, Löhr F, Tumulka F, Reichel K, Würz J, Hummer G, Schäfer L, Tampé R, Joseph B, Bernhard F, Dötsch V, Abele R. Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL. Sci. Rep., 8(1), 15662, https://doi.org/10.1038/s41598-018-33841-w

2018

  • Barth K, Hank S, Spindler PE, Prisner TF, Tampé R, Joseph B. Conformational Coupling and trans-Inhibition in the Human Antigen Transporter Ortholog TmrAB Resolved with Dipolar EPR Spectroscopy. J. Am. Chem. Soc., 140, 4527–4533, https://doi.org/10.1021/jacs.7b12409

2017

  • Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM,Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, StroudRM, Pos KM, and Tampé R. Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP. Proc. Natl. Acad. Sci. USA., 114, E438-447. https://doi.org/10.1073/pnas.1620009114

2016

  • Sikora A, Joseph B, Matson M, Staley JR, Cafiso DS. Allosteric Signaling Is Bidirectional in an Outer-Membrane Transport Protein. Biophys. J., 111,1908-18, https://doi.org/10.1016/j.bpj.2016.09.038
  • Joseph B*, Tormyshev VM, Rogozhnikova OYu, Akhmetzyanov D, Bagryanskaya EG, Prisner TF*. Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes using Trityl - Nitroxide PELDOR. Angew. Chem. Int. Ed., 55,11538-42, https://doi.org/10.1002/anie.201606335
  • Joseph B*, Sikora A, Cafiso DS*. Ligand induced conformational changes in a membrane transporter in E. coli cell’s observed with DEER/PELDOR. J. Am. Chem. Soc., 138, 1844-47, (highlighted in JACS Spotlights) https://doi.org/10.1021/jacs.5b13382

2015

  • Joseph B, Sikora A, Bordignon E, Jeschke G, Cafiso DS, Prisner TF. Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy. Angew. Chem. Int. Ed., 54, 6196-6199https://doi.org/10.1002/anie.201501086

2014

  • Joseph B, Korkhov VM, Yulikov M, Jeschke G, Bordignon E. Conformational cycle of the vitamin B12 ABC importer in liposomes detected by double electron-electron resonance (DEER).  J. Biol. Chem., 289, 3176-3185, https://doi.org/10.1074/jbc.M113.512178

2012

  • Doll A, Bordignon E, Joseph B, Tschaggelar R, Jeschke G. Liquid state DNP for water accessibility measurements on spin-labeled membrane proteins at physiological temperatures. J. Magn. Reson., 222, 34-43, https://doi.org/10.1016/j.jmr.2012.06.003

2011

  • Joseph B, Jeschke G, Goetz BA, Locher KP, Bordignon E. Transmembrane gate movements in the type II ATP-binding cassette (ABC) importer BtuCD-F during nucleotide cycle. J. Biol. Chem., 286, 41008-41017, https://doi.org/10.1074/jbc.M111.269472

2008

  • Kajiyama S, Joseph B, Inoue F, Shimamura M, Fukusaki E, Tomizawa K, Kobayashi A (2008) Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation.  J. Biosci. Bioeng., 106, 194-198, https://doi.org/10.1263/jbb.106.194