Seminarraum 1.1.33, Mo 10:00-12:00
26.3.2012-30.3.2012 from 9:00 am-18:00 pm
Zielgruppe: Master students, diploma strudents and PhD students with interest in generating their biological samples by themselves for biophysical investigations.
Lectures about basics e.g. how to mutagenise a protein, cloning techniques, expression and purification of proteins and the underlying cellular processes when expressing a protein will be given prior to the practical exercises in the lab. Practical exercises : -molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein -cultivation of bacteria to express the protein -purification of membrane proteins by affinity chromatography -amplification of DNA by PCR (Polymerase chain reaction), analysis of DNA with enzymes and agarose-gel-electrophoresis and analysis of proteins on SDS-gels.