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Publications

2023

  • Balke, J., DíazGutiérrez, P., Rafaluk-Mohr, T., Proksch, J., Koksch, B. and Alexiev, U.
    Osmolytes Modulate Photoactivation of Phytochrome: Probing Protein Hydration
    Molecules, in press
  • Alexiev, U. and Rühl, E.,
    Visualization of Nanocarriers and Drugs in Cells and Tissue
    Spinger, Handbook of experimental Pharmacology, 2023 doi:10.1007/164_2023_684
  • Wolf, A., Volz-Rakebrand, P., Balke, J. and Alexiev, U.
    Diffusion Analysis of NAnoscopic Ensembles: A Tracking-Free Diffusivity Analysis for NAnoscopic Ensembles in Biological Samples and Nanotechnology
    Small, 19, 2206722, doi: 10.1002/smll.202206722

2022

  • Sadeghi, M., Balke, J., Rafaluk-Mohr, T. and Alexiev, U.
    Long-Distance Protonation-Conformation Coupling in Phytochrome Species
    Molecules, 27, 23: 8395, 2022 doi: 10.3390/molecules27238395
  • Silapetere, A., Hwang, S., Hontani, Y., Fernandez Lahore, R.G., Balke, J., Escobar, F.V., Tros, M., Konold, P.E., Matis, R., Croce, R., Walla, P.J., Hildebrandt, P., Alexiev, U., Kennis, J.T.M., Sun, H., Utesch, T. and Hegemann, P.
    QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins
    Nat. Comm., 13, 1: 5501, 2022 doi: 10.1038/s41467-022-33084-4
  • Priscila Schilrreff and Ulrike Alexiev.
    Chronic Inflammation in Non-Healing Skin Wounds and Promising Natural Bioactive Compounds Treatment
    International Journal of Molecular Sciences, 23, 4928, 2022 doi: 10.3390/ijms23094928
  • Soshichiro Nagano, Maryam Sadeghi, Jens, Balke,Moritz Fleck, Nina Heckmann, Georgios Psakis and Ulrike Alexiev. Improved fluorescent phytochromes for in situ imaging.
    Scientific Reports, 12, 5587, 2022 doi: 10.1038/s41598-022-09169-x
  • Anastasia I. Govdi, Polina V. Tokareva, Andrey M. Rumyantsev, Maxim S. Panov, Johannes Stellmacher, Ulrike Alexiev, Natalia A. Danilkina and Irina A. Balova.
    4,5-Bis(arylethynyl)-1,2,3-triazoles—A New Class of Fluorescent Labels: Synthesis and Applications
    Molecules, 27, 3191, 2022, doi: 10.3390/molecules27103191

2021

  • Pin Dong, Johannes Stellmacher, Lydia M. Bouchet, Marius Nieke, Amit Kumar, Ernesto R. Osorio-Blanco, Gregor Nagel, Silke B. Lohan, Christian Teutloff, Alexa Patzelt, Monika Schäfer-Korting, Marcelo Calderón, Martina C. Meinke, and Ulrike Alexiev.
    A Dual Fluorescence-Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM – EPR Spectroscopy
    Angewandte Chemie, 60, 14938, 2021 doi: 10.1002/anie.202012852

2020

    • Leonie Gronbach, Christopher Wolff, Konrad Klinghammer, Johannes Stellmacher, Philipp Jurmeister, Ulrike Alexiev, Monika Schäfer-Korting, Ingeborg Tinhofer, Ulrich Keilholz, Christian Zoschke.
      A multilayered epithelial mucosa model of head neck squamous cell carcinoma for analysis of tumor-microenvironment interactions and drug development.
      Biomaterials, 258,120277, 2020 doi:10.1016/j.biomaterials.2020.120277

    • Robert Brodwolf, Pierre Volz-Rakebrand, Johannes Stellmacher, Christopher Wolff, Michael Unbehauen, Rainer Haag, Monika Schäfer-Korting, Christian Zoschke, Ulrike Alexiev.
      Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue.
      Theranostics , 11(14), 6322-6336, 2020 doi:10.7150/thno.42581

    • Alexander Wolf, Jovan Dragelj, Juliane Wonneberg, Johannes Stellmacher, Jens Balke, Anna Lena Woelke, Milan Hodoscek, Ernst Walter Knapp and Ulrike Alexiev
      The redox-coupled proton-channel opening in cytochrome c oxidase
      ChemicalScience, 11, 3804-3811, 2020 doi:10.1039/C9SC06463J

    • Maryam Sadeghi, Jens Balke, Constantin Schneider, Soshichiro Nagano, Johannes Stellmacher, Günter Lochnit, Christina Lang, Chris Weise, Jon Hughes, Ulrike Alexiev,
      Transient Deprotonation of the Chromophore Affects Protein Dynamics Proximal and Distal to the Linear Tetrapyrrole Chromophore in Phytochrome Cph1
      Biochemistry, 2020 doi:10.1021/acs.biochem.9b00967

2019

    • Alexander Wolf, Juliane Wonneberg, Jens Balke and Ulrike Alexiev,
      Electronation-dependent structural change at the proton exit side of cytochrome c oxidase as revealed by site-directed fluorescence labeling
      FEBS J, 2019 doi: 10.1111/febs.15084

    • Katja Ober, Pierre Volz-Rakebrand, Johannes Stellmacher, Robert Brodwolf, Kai Licha, Rainer Haag and Ulrike Alexiev,
      Expanding the Scope of Reporting Nanoparticles: Sensing of Lipid Phase Transitions and Nanoviscosities in Lipid Membranes,
      Langmuir 35 (35), 11422-11434, 2019 doi: 10.1021/acs.langmuir.9b01372

    • Janna Frombach, Michael Unbehauen, Indah N. Kurniasih, Fabian Schumacher, Pierre Volz, Sabrina Hadam, Fiorenza Rancan, Ulrike Blume-Peytavi, Burkhard Kleuser, Rainer Haag, Ulrike Alexiev and Annika Vogt,
      Core-multishell nanocarriers enhance drug penetration and reach keratinocytes and antigen-presenting cells in intact human skin,
      J Control Release 299, 138-148, 2019 doi: 10.1016/j.jconrel.2019.02.028

2018

    • Jens Balke, Pierre Volz, Falko Neumann, Robert Brodwolf, Alexander Wolf, Hannah Pischon, Moritz Radbruch, Lars Mundhenk, Achim D. Gruber, Nan Ma and Ulrike Alexiev,
      Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range using Fluorescence Lifetime Imaging
      Small 14 (23), 18700106, 2018 doi: 10.1002/smll.201800310

    • Alexander Edlich, Pierre Volz, Robert Brodwolf, Michael Unbehauen, Lars Mundhenk, Achim D. Gruber, Sarah Hedtrich, Rainer Haag, Ulrike Alexiev, Burkhard Kleuser,
      Crosstalk between core-multishell nanocarriers for cutaneous drug delivery and antigen-presenting cells of the skin
      Biomaterials 162, 60-70, 2018 doi: 10.1016/j.biomaterials.2018.01.058

    • Pierre Volz, Robert Brodwolf, Christian Zoschke, Rainer Haag, Monika Schäfer-Korting and Ulrike Alexiev
      White-Light Supercontinuum Laser-Based Multiple Wavelength Excitation for TCSPC-FLIM of Cutaneous Nanocarrier Uptake
      Z. Phys. Chem. , 2018 doi: 10.1515/zpch-2017-1050


    • N Döge, S Hadam, P Volz, A Wolf, KH Schönborn, U Blume-Peytavi, U Alexiev, A Vogt.
      Identification of polystyrene nanoparticle penetration across intact skin barrier as rare event at sites of focal particle aggregations.
      J Biophotonics
      2018 Apr;11(4):e201700169. doi.org/10.1016/j.ejpb.2016.10.021


2017

    • P Volz, P Schilrreff, R Brodwolf, C Wolff, J Stellmacher, J Balke, MJ Morilla, C Zoschke, M Schäfer-Korting, U Alexiev.
      Pitfalls in using fluorescence tagging of nanomaterials: tecto-dendrimers in skin tissue as investigated by Cluster-FLIM.
      Ann N Y Acad Sci. 1405(1), 202-214, 2017. doi: 10.1111/nyas.13473/s11671-017-1835-0


    • M Radbruch, H Pischon, A Ostrowski, P Volz, R Brodwolf, F Neumann, M Unbehauen, B Kleuser, R Haag, N Ma, U Alexiev, L Mundhenk, A Gruber.
      Dendritic Core-Multishell Nanocarriers in Murine Models of Healthy and Atopic Skin.
      Nanoscale Res Lett 12(64), 2017. doi: 10.1186/s11671-017-1835-0


    • U Alexiev, P Volz, A Boreham, R Brodwolf.
      Time-resolved fluorescence microscopy (FLIM) as an analytical tool in skin nanomedicine.
      Eur J Pharm Biopharm. 116, 111-124, 2017. doi: 10.1016/j.ejpb.2017.01.005


    • L Lowenau, C Zoschke ,R Brodwolf, P Volz, C Hausmann, S Wattanapitayakul, A Boreham, U Alexiev, M Schafer-Korting.
      Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes.
      Eur J Pharm Biopharm. 116, 149-154, 2017. doi: 10.1016/j.ejpb.2016.12.017


    • A Boreham, R Brodwolf, K Walker, R Haag, U Alexiev.
      Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine.
      Molecules 22 (1), 17, 2017, doi:10.3390/molecules22010017


    • F Velazquez Escobar, C Lang, A Takiden, C Schneider, J Balke, J Hughes, U Alexiev, P Hildebrandt , M Mroginski.
      Protonation-Dependent Structural Heterogeneity in the Chromophore Binding Site of Cyanobacterial Phytochrome Cph1.
      J. Phys. Chem. B, 2017, 121 (1), 47–57 DOI: 10.1021/acs.jpcb.6b09600


    • A Boreham, P Volz, D Peters, C. Keck, U Alexiev.
      Determination of nanostructures and drug distribution in lipid nanoparticles by single molecule microscopy.
      Eur J Pharm Biopharm 110, 31-38, 2017. doi: 10.1016/j.ejpb.2016.10.020


    • H Pischon, M Radbruch, A Ostrowski, P Volz, C Gerecke, M Unbehauen, S Hönzke, S Hedtrich, J Fluhr, R Haag, B Kleuser, U Alexiev, A Gruber, L Mundhenk.
      Stratum corneum targeting by dendritic core-multishell-nanocarriers in a mouse model of psoriasis.
      Nanomedicine 13(1), 317-327, 2017. DOI: 10.1016/j.nano.2016.09.004


    • G Zoubari, S Staufenbiel, P Volz, U Alexiev, R Bodmeier.
      Effect of drug solubility and lipid carrier on drug release from lipid nanoparticles for dermal delivery.
      Eur J Pharm Biopharm
      , 110, 39-46, 2017. doi.org/10.1016/j.ejpb.2016.10.021


    • N Zhang, A Said, C Wischke, V Kral, R Brodwolf, P Volz, A Boreham, C Gerecke, W Li, A Neffe, B Kleuser, U Alexiev, A Lendlein, M Schäfer-Korting.
      Poly[acrylonitrile-co-(N-vinyl pyrrolidone)] nanoparticles -composition-dependent skin penetration enhancement of a dye probe andbiocompatibility.
      Eur J Pharm Biopharm. 116, 66-75, 2017. doi: 10.1016/j.ejpb.2016.10.019

2016

    • A Vogt,C Wischke, AT Neffe, N Ma, RH Müller, U Alexiev, A Lendlein.
      Nanocarriers for drug delivery into and through the skin: Do existing technologies match clinical challenges?
      J. Contr. Release. 242, 3-15, 2016. doi: 10.1016/j.jconrel.2016.07.027


    • P Volz, N Krause, J Balke, C Schneider, M Walter, F Schneider, R Schlesinger, U Alexiev.
      Light and pH-induced changes in structure and accessibility of transmembrane helix B and its immediate environment in Channelrhodopsin-2.
      J. Biol. Chem. 291, 33, 17382-17393, 2016. doi: 10.1074/jbc.M115.711200


    • A Wolf, C Schneider, TY Kim, K Kirchberg, P Volz, U Alexiev.
      A simulation-guided fluorescence correlation spectroscopy tool to investigate to protonation dynamics of cytrochrome c oxidase.
      Phys. Chem. Chem. Phys.18, 12877-12885, 2016. doi: 10.1039/c5cp07925j.


    • RH Müller, U Alexiev, P Sinambela, CM Keck.
      Nanostructured Lipid Carriers (NLC): The Second Generation of Solid Lipid Nanoparticles. In: Percutaneous Penetration Enhancer Chemical Methods in Penetration Enhancement: Nanocarriers, Eds. Nina Dragicevic, Howard I. Maibach (Springer). 2016


    • A Boreham, J Pikkemaat, P Volz, R Brodwolf, C Kuehne, K Licha, R Haag, J Dernedde, U Alexiev.
      Detecting and Quantifiying Biomolecular Interactions of a Dendritic Polyglycerol Sulfate Nanoparticle Using Fluorescence Lifetime Measurements.
      Molecules
      21(1), 22. 2016. doi:10.3390/molecules21010022


2015

    • C Richter, C Schneider, MT Quick, P Volz, R Mahrwald, J Hughes, B Dick, U Alexiev, NP Ernsting.
      Dual-fluorescence pH probe for bio-labelling.
      Phys. Chem. Chem. Phys. 2015. doi: 10.1039/c5cp05454k


    • M Witting, A Boreham, R Brodwolf, K Vavrova, U Alexiev, W Friess, S. Hedtrich.
      Interactions of Hyaluronic Acid with the Skin and Implications for the Dermal Delivery of Biomacromolecules.
      Molecular Pharmaceutics. 2015. doi: 10.1021/mp500676e


    • P Volz, A Boreham, A. Wolf, TY Kim, J Balke, J Frombach, S Hadam, Z Afraz, F Rancan, U Blume-Peytavi, A Vogt, U Alexiev.
      Application of Single Molecule Fluorescence Microscopy to Characterize the Penetration of a Large Amphiphilic Molecule in the Stratum Conerum of Human Skin.
      International Journal of Molecular Sciences
      . 16(4), 6960-69772015. doi:10.3390/ijms16046960


    • A Ostrowski, D Nordmeyer, A Boreham, C Holzhausen, L Mundhenk, C Graf, MC Meinke, A Vogt, S Hadam, J Lademann, E Rühl, U Alexiev, AD Gruber.
      Overview on the Localization of Nanoparticles in Tissue and Cellular Context by Different Imaging Techniques.
      Beilstein Journal of Nanotechnology 6: 263-80. 2015. doi:10.3762/bjnano.6.25


    • YJ Zhu, CS Choe, S Ahlberg, MC Meinke, U Alexiev, J Lademann, ME Darvin.
      Penetration of silver nanoparticles into porcine skin ex vivo using fluorescence lifetime imaging microscopy, Raman microscopy and surface enhanced Raman scattering microscopy.
      J. Biomed. Opt. 2015. doi:10.1117/1.JBO.20.5.051006


    • J Lademann, F Knorr, H Richter, S Jung, MC Meinke, E Rühl, U Alexiev, M Calderon, A Patzelt.
      Hair follicles as a target structure for nanoparticles.
      Journal of Innovative Optical Health Sciences
      . 2015. doi: 10/1142/S1793545815300049


2014

    • A Boreham, R Brodwolf, M Pfaff, T-Y Kim, T Schlieter, L Mundhenk, AD Gruber, D Gröger, K Licha, R Haag, and U Alexiev. Temperature and environment dependent dynamic properties of a dendritic polyglycerol sulfate.
      Polym. Adv. Technol. 2014. doi: 10.1002/pat.3355



    • U Alexiev, DL Farrens.
      Fluorescence spectroscopy of rhodopsins: Insights and approaches.
      Biochim Biophys Acta.
      2014 May. 1837(5), SI: 694-709.


      A Ostrowski, D Nordmeyer, A Boreham, R Brodwolf, L Mundhenk, JW Fluhr, J Lademann, C Graf, E Rühl, U Alexiev, AD Gruber. Skin barrier disruptions in tape stripped and allergic dermatitis models have no effect on dermal penetration and systemic distribution of AHAPS-functionalized silica nanoparticles.
      Nanomedicine
      . 2014 Apr 22. pii: S1549-9634(14)00187-7. doi: 10.1016/j.nano.2014.04.004.


    • N Alnasif, C Zoschke, E Fleige, R Brodwolf, A Boreham, E Rühl, KM Eckl, HF Merk, HC Hennies, U Alexiev, R Haag, S Küchler, M Schäfer-Korting. Penetration of normal, damaged and diseased skin - An in vitro study on dendritic core-multishell nanotransporters.
      J Control Release. 2014 Jul 10;185:45-50. doi: 10.1016/j.jconrel.2014.04.006. Epub 2014 Apr 13.


    • A Boreham, M Pfaff, E Fleige, R Haag, U Alexiev., Nanodynamics of dendritic core-multishell nanocarriers.
      Langmuir
      . 2014 Feb 18;30(6):1686-95. doi: 10.1021/la4043155. Epub 2014 Feb 5.



2013

  • C Hoppmann, U Alexiev, E Irran, K Rück-Braun.
    Synthesis and fluorescence of xanthone amino acids,
    Tetrahedron Letters
    , 2013 Aug 21, 54, 34, 4585-4587

  • K Kirchberg, H Michel, U Alexiev.
    Exploring the entrance of proton pathways in cytochrome c oxidase from Paracoccus denitrificans: surface charge, buffer capacity and redox-dependent polarity changes at the internal surface.
    Biochim Biophys Acta.
    2013 Mar;1827(3):276-84

  • U Alexiev.
    Dynamics of Helix 8 in GPCR Function.
    Encyclopedia of Biophysics
    , 978-3-642-16711-9, 2013, pp 549-552


  • CM Keck, A Boreham, T Schlieter, D Peters, RH Müller, U Alexiev.
    Nanostructures and drug distribution within Nanostructured Lipid Carriers (NLC), In: “Proceedings of the 40th Annual Meeting of the Controlled Release Society (CRS)” ID 100536 (2013).

2012

  • B. Repen, E. Schneider, and U. Alexiev.
    Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins.
    Anal. Biochemistry
    , 2012, 2012 Jul 15;426(2):103-5
  • K. Kirchberg, H. Michel, and U. Alexiev.
    Net Proton Uptake Is Preceded by Multiple Proton Transfer Steps upon Electron Injection into Cytochrome c Oxidase. J. Biol. Chem., 2012, 287, 8187-93
  • T.-Y. Kim , T. Schlieter , S. Haase, and U. Alexiev.
    Activation and molecular recognition of the GPCR rhodopsin - Insights from time-resolved fluorescence depolarisation and single molecule experiments.
    Eur J Cell Biol.
    , 2012, 91, 300-310

2011

  • A. Boreham, T.-Y. Kim, V. Spahn, C. Stein, L. Mundhenk, A. D. Gruber, R. Haag, P. Welker, K. Licha, and U. Alexiev.
    Exploiting fluorescence lifetime plasticity in FLIM: Target molecule localisation in cells and tissues.
    ACS Med. Chem. Lett., 2011, 2 (10), 724–728
  • K. Kirchberg, T.-Y. Kim, M. Möller, D. Skegro, G. Dasara Raju, J. Granzin, G. Büldt, R. Schlesinger, and U. Alexiev.
    Conformational dynamics of helix-8 in the GPCR rhodopsin controls arrestin activation in the desensitization process.
    PNAS
    , 2011, 108 (46), 18690-18695

2010

  • A. Dutta, K. C. Tirupula, U. Alexiev, and J. Klein-Seetharaman.
    Characterization of membrane protein non-native states. 1. Extent of unfolding and aggregation of rhodopsin in the presence of chemical denaturants.
    Biochemistry
    , 2010, 49, 6317-6328

  • A. Dutta, T.-Y. Kim, M. Moeller, J. Wu, U. Alexiev, and J. Klein-Seetharaman.
    Characterization of membrane protein non-native states. 2. The SDS-unfolded states of rhodopsin.
    Biochemistry
    , 2010, 49, 6329-6340

  • K. Kirchberg, T.-Y. Kim, S. Haase, and U. Alexiev.
    Functional interaction structures of the photochromic retinal protein rhodopsin. Photochem.
    Photobiol. Sci.
    , 2010, 9, 226-33

2009

2008

  • D. Narzi, K. Winkler, J. Saidowsky, R. Misselwitz, A. Ziegler, R.A. Böckmann, and U. Alexiev
    Molecular determinants of MHC class I complex stability: Shaping antigenic features through long-range electrostatic interactions.
    J. Biol. Chem. 2008, 283, 23093-23103

2007

  • K. Winkler, A. Winter, C. Rückert, B. Uchanska-Ziegler, and U. Alexiev.
    Natural MHC Class I Polymorphism Controls the Pathway of Peptide Dissociation from HLA-B27 complexes.
    Biophys. J., 2007, 93(8), 2743-55

  • K. Winkler, J. Saidowsky, A. Ziegler, and U. Alexiev.
    Molecular determinants of MHC class I stability.
    In:International Proceedings of the 13th International Congress of Immunology (Kalil, J. et al eds.), Medimont Bologna (2007) ISBN 978-88-7587-379-0

  • T.-Y. Kim, K. Winkler, and U. Alexiev.
    Picosecond Multi-dimensional Fluorescence Spectroscopy: A tool to measure real-time protein dynamics during function.
    Photochemistry and Photobiology 83, 378-384 (2007)

  • N. Lehmann, U. Alexiev, and K. Fahmy.
    Linkage Between the Intramembrane H-bond Network Around Aspartic Acid83 and the Cytosolic Environment of Helix 8 in Photoactivated Rhodopsin.
    J. Mol. Biol. 366, 1129-1141 (2007)

2006

  • B. Uchanska-Ziegler, U. Alexiev, R. Hillig, M. Hülsmeyer, T. Pöhlmann, W. Saenger, A. Volz, and A. Ziegler.
    X-ray crystallography and dynamic studies of HLA-B*2705 and B*2709 molecules complexed with the same peptide.
    In: Immunobiology of the Human MHC: Proceedings of the 13th International Histocompatibility Workshop and Conference (Hansen, J.A., ed.), IHWG Press, Seattle (2006) ISBN:0-945278-03-9

2005

  • G. Schröder, U. Alexiev, and H. Grubmüller.
    Simulation of fluorescence anisotropy experiments: Probing protein dynamics.
    Biophys. J. 89(6), 3757-70 (2005)

  • A. Selle, C. Kappelt, M. A. Bader, G. Marowsky, K. Winkler, U. and Alexiev.
    Picosecond pulse induced two-photon fluorescence enhancement in biological material applying grating waveguide structures.
    Optics Lett. 30, 1683-1685 (2005)

  • V. Jaakola, M. Rehn, M. Möller, U. Alexiev, A. Goldman, and G. J. Turner.
    G-protein-coupled receptor domain over-expression in Halobacterium salinarium: Long-range allosteric interactions from the intracellular to the extracellular side in heptahelical membrane proteins.
    Proteins 60, 412-23 (2005)

  • M. Hülsmeyer, K. Welfle, T. Pöhlmann, R. Misselwitz, U. Alexiev, H. Welfle, W. Saenger, B. Uchanska- Ziegler, and A. Ziegler.
    Thermodynamic and structural equivalence of two HLA-B27 subtypes complexed with a Self-peptide.
    J. Mol. Biol. 346, 1367-1379 (2005)

2004

  • T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev.
    Differential peptide dynamics is linked to MHC polymorphism.
    J. Biol. Chem. 279, 28197-28201 (2004)

2003

  • U. Alexiev, I. Rimke, and T. Pöhlmann.
    Elucidation of the nature of the conformational changes of the EF-interhelical loop in bacteriorhodopsin and of the helix VIII on the cytoplasmic surface of rhodopsin: A time-resolved fluorescence depolarization study.
    J. Mol. Biol. 328, 705-719 (2003)

  • B. Uchanska-Ziegler, U. Alexiev, R. Hillig, M. Hülsmeyer, T. Pöhlmann, W. Saenger, A. Volz, and A. Ziegler (2003).
    In HLA 2002. The immunobiology of the human MHC. Eds. Dupont B, Hansen J. IHWG Press, Seattle

2002

  • T. Mielke, U. Alexiev, M. Gläsel, H. Otto, and M. P. Heyn.
    Light-induced changes in the structure and accessibility of the cytoplasmic loops of rhodopsin in the activated MII-state.
    Biochemistry 41, 7875-84 (2002)

  • R. A. Krebs, U. Alexiev, R. Parthasarathy, A.-M. DeVita, and M. S. Braiman.
    Detection of light-activated H+-release and M-intermediate formation from purified proteorhodopsin.
    BMC Physiology 2 (2002)

2000

  • U. Alexiev, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn.
    Evidence for long range allosteric interactions between the extracellular and cytoplasmic parts of bacteriorhodopsin from the mutant R82A and its second site revertant R82A/G231C.
    J. Biol. Chem. 275, 13431-13440 (2000)

  • K. Heyne, J. Herbst, B. Dominguez Herradon, U. Alexiev, and R. Diller.
    Reaction control in bacteriorhodopsin: Impact of Arg82 and Asp85 on the fast retinal isomerization, studied in the second site revertant Arg82Ala/Gly231Cys and various purple and blue forms of bacteriorhodopsin.
    Phys. Chem. B 104, 6053-6058 (2000)

  • M. S. Hutson, U. Alexiev , S. V. Shilov, K. J. Wise, and M. S. Braiman.
    Evidence for a perturbation of Arginine-82 in the bacteriorhodopsin photocycle from Time-­resolved Infrared spectra.
    Biochemistry 39, 13189-13200 (2000)

1998

  • W. Behrens, U. Alexiev, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn
    Structure of the interhelical loops and carboxyl terminus of bacteriorhodopsin by X-ray diffraction using site-directed heavy-atom labeling.
    Biochemistry 37, 10411-10419 (1998)

  • K. Heyne, R. Dziewior, R. Diller, and U. Alexiev.
    Single and double mutants of bacteriorhodopsin and their impact on photoisomerization.
    In Springer Series in Chemical Physics: "Ultrafast phenomena XI". Eds. T. Elsaesser, J.G. Fujimoto, D. A. Wiersma, W. Zinth, 681-683 (1998)

1996

  • S. Moltke, U. Alexiev, and M. P. Heyn.
    Kinetics of light-induced intramolecular charge transfer and proton release in bacteriorhodopsin.
    Israel. J. Chem. 35, 235-248 (1996)

1995

  • U. Alexiev, R. Mollaaghababa, P. Scherrer, H. G. Khorana, and M. P. Heyn.
    Rapid long ­range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.
    PNAS 92, 372-376 (1995)

  • U. Alexiev, P. Scherrer, T. Marti, H. G. Khorana, and M. P. Heyn.
    Time-resolved surface charge change on the cytoplasmic side of bacteriorhodopsin.
    FEBS Letters 373, 81-84 (1995)

  • S. Dickopf, U. Alexiev, M. P. Krebs, H. Otto, R. Mollaaghababa, H. G. Khorana, and M. P. Heyn.
    Proton transport by a bacteriorhodopsin mutant, aspartic acid-85—> asparagine, initiated in the unprotonated Schiff base state.
    PNAS 92, 11519-11523 (1995)

1994

  • U. Alexiev, T. Marti, M. P. Heyn, H. G. Khorana, and P. Scherrer.
    Surface charge of bacteriorhodopsin detected with covalently bound pH-indicators at selected extracellular and cytoplasmic sites.
    Biochemistry 33, 298 - 306 (1994)

  • P. Scherrer, U. Alexiev, T. Marti, H. G. Khorana, and M. P. Heyn.
    Covalently bound pH­indicator dyes at selected extracellular or cytoplasmic sites in bacteriorhodopsin. I. Light­induced proton release kinetics and proton migration along the surface of bacteriorhodopsin micelles.
    Biochemistry 33, 13684-13699 (1994)

  • U. Alexiev, T. Marti, M. P. Heyn, H. G. Khorana, and P. Scherrer.
    Covalently bound pH­indicator dyes at selected extracellular or cytoplasmic sites in bacteriorhodopsin. II. Rotational orientation of helices D and E and kinetic correlation between M-formation and proton release in bacteriorhodopsin micelles.
    Biochemistry 33, 13693-13699 (1994)

1992

  • P. Scherrer, U. Alexiev, M. P. Heyn, T. Marti, and H.G. Khorana.
    Proton movement and surface charge in bacteriorhodopsin detected by selectively attached pH-indicators.
    In Structures and Functions of Retinal Proteins. Ed. J.L. Rigaud. Colloque INSERM/John Libbey Eurotext Ltd. Vol. 221: 205 - 211 (1992)

  • D. A. Greenhalgh, S. Subramaniam, U. Alexiev, H. Otto, M. P. Heyn, and H. G. Khorana.
    Effect of introducing different carboxylate-containing side chains at position 85 on chromophore formation and proton transport in bacteriorhodopsin.
    J. Biol. Chem. 267, 25734-25738 (1992)