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Publikationen

Paschke RR, Mohr S, Lange S, Lange A, Kozuch J
In Situ Spectroscopic Detection of Large-Scale Reorientations of Transmembrane Helices During Influenza A M2 Channel Opening
Angew. Chem.Int. Ed.2023,62, e2023090     doi.org/10.1002/anie.202309069


González-Viegas M, Kar RK, Miller A-F, and Mroginski M-A
Noncovalent interactions that tune the reactivities of the flavins in bifurcating electron transferring flavoprotein
J. Biol. Chem. 2023 299(6) 104762   doi.org/10.1016/j.jbc.2023.104762


Flores-Ibarra A, Maia RNA, Olasz B, Church JR, Gotthard G, Schapiro I, Heberle J, Nogly P
Light-Oxygen-Voltage (LOV)-sensing domains: activation mechanism and optogenetic stimulation
J Mol Biol. 2023 Nov 7:168356. doi: 10.1016/j.jmb.2023.168356. Online ahead of print.


Schubert L, Chen JL, Fritz T, Marxer F, Langner P, Hoffmann K, Gamiz-Hernandez AP, Kaila VRI, Schlesinger R, Heberle J
Proton Release Reactions in the Inward H+ Pump NsXeR
J Phys Chem B. 2023 Oct 5;127(39):8358-8369. doi: 10.1021/acs.jpcb.3c04100. Epub 2023 Sep 20.


Bühl E, Resler T, Lam R, Asido M, Bamberg E, Schlesinger R, Bamann C, Heberle J, Wachtveitl J
Assessing the Role of R120 in the Gating of CrChR2 by Time-Resolved Spectroscopy from Femtoseconds to Seconds
J Am Chem Soc. 2023 Sep 29. doi: 10.1021/jacs.3c05399. Online ahead of print.


Kozuch J, Ataka K, Heberle J
Surface-enhanced infrared absorption spectroscopy
Nature Rev. Meth. Prim. 3, Article number: 70 (2023).   DOI: 10.1038/s43586-023-00253-8


Saliminasab M, Yamazaki Y, Palmateer A, Harris A, Schubert L, Langner P, Heberle J, Bondar AN, Brown LS
A Proteorhodopsin-Related Photosensor Expands the Repertoire of Structural Motifs Employed by Sensory Rhodopsins
J Phys Chem B. 2023 Sep 21;127(37):7872-7886. doi: 10.1021/acs.jpcb.3c04032. Epub 2023 Sep 11.PMID: 37694950


Aleksanyan M, Grafmüller A, Crea F, Georgiev VN, Yandrapalli N, Block S, Heberle J, Dimova R
Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene-Lipid Photoswitches
Adv Sci (Weinh). 2023 Aug 31:e2304336. doi: 10.1002/advs.202304336. Online ahead of print.PMID: 37653602


Baserga F, Storm J, Schlesinger R, Heberle J, Stripp ST
The catalytic reaction of cytochrome c oxidase probed by in situ gas titrations and FTIR difference spectroscopy
Biochim Biophys Acta Bioenerg. 2023 Nov 1;1864(4):149000. doi: 10.1016/j.bbabio.2023.149000. Epub 2023 Jul 27

Hohmann T, Chowdhary S, Ataka K, Er J, Dreyhsig G H, Heberle J,  Koksch B
Introducing Aliphatic Fluoropeptides: Perspectives on Folding Properties, Membrane Partition and Proteolytic Stability
Chem. Eur. J. 2023, e202203860.   DOI: 10.1002/chem.202203860





Soboh B, Adrian L, Stripp S T
An in vitro reconstitution system to monitor iron transfer to the active site during the maturation of [NiFe]-hydrogenase
J. Biol. Chem. (2022) 298(9) 102291.  doi.org/10.1016/j.jbc.2022.102291


Stripp S T, Duffus B R, Fourmond V, Léger C, Leimkühler S, Hirota S, Hu Y, Jasniewski A, Ogata H, and Ribbe M W
Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase
Cite This: Chem. Rev. 2022, 122, 11900−11973.  doi.org/10.1021/acs.chemrev.1c00914


Senger M, Duan J, Pavliuk M V, Apfel U-P, Haumann M, and Stripp S T
Trapping an Oxidized and Protonated Intermediate of the [FeFe]-Hydrogenase Cofactor under Mildly Reducing Conditions
Inorg. Chem. 2022, 61, 26, 10036–10042.   doi.org/10.1021/acs.inorgchem.2c00954


Ji Z, Kozuch J, Mathews I I, Diercks C S , Shamsudin Y, Schulz M A, Boxer S G
Protein electric fields enable faster and longer-lasting covalent inhibition of beta-lactamases
J. Am. Chem. Soc., 2022, 144, 20947-20954. doi.org/10.1021/jacs.2c09876


Utesch T, Staffa J K, Katz S, Yao G, Kozuch J, Hildebrandt P
Potential distribution across model membranes
J. Phys. Chem. B, 2022. 126, 7664-7675.  doi.org/10.1021/acs.jpcb.2c05372


Walter M, Schubert L, Heberle J, Schlesinger R, Losi A
Time-resolved photoacoustics of channelrhodopsins: early energetics and light-driven volume changes

Photochem Photobiol Sci. 2022 Oct 23. doi: 10.1007/s43630-022-00327-8. Online ahead of print


Fernandes R, Chowdhary S, Mikula N, Saleh N, Kanevche K, von Berlepsch H, Hosogi N, Heberle J, Weber M, Böttcher C, Koksch B
Cyanine Dye Coupling Mediates Self-assembly of a pH Sensitive Peptide into Novel 3D Architectures
Angew Chem Int Ed Engl. 2022 Sep 26.   doi: 10.1002/anie.202208647. Online ahead of print.


Ataka K, Baumann A, Chen JL, Redlich A, Heberle J, Schlesinger R
Monitoring the progression of cell-free expression of microbial rhodopsins by Surface Enhanced IR spectroscopy: Resolving a branch point for successful/unsuccessful folding
Front Mol Biosci. 2022 July 14;9:929285.   doi: 10.3389/fmolb.2022.929285


Baserga F, Vorkas A, Crea F, Schubert L, Chen JL, Redlich A, La Greca M, Storm J, Oldemeyer S, Hoffmann K, Schlesinger R, Heberle J
Membrane Protein Activity Induces Specific Molecular Changes in Nanodiscs Monitored by FTIR Difference Spectroscopy
Front Mol Biosci. 2022 Jun 13;9:915328. doi: 10.3389/fmolb.2022.915328


Crea F, Vorkas A, Redlich A, Cruz R, Shi C, Trauner D, Lange A, Schlesinger R, Heberle J
Photoactivation of a Mechanosensitive Channel
Front. Mol. Biosci., 28 June 2022 | https://doi.org/10.3389/fmolb.2022.905306


Schubert L, Langner P, Ehrenberg D, Lorenz-Fonfria VA, Heberle J
Protein conformational changes and protonation dynamics probed by a single shot using quantum-cascade-laser-based IR spectroscopy
J Chem Phys. 2022 May 28;156(20):204201,  doi: 10.1063/5.0088526


Weaver J B, Kozuch J, Kirsh J M, Boxer S G
Nitrile IR intensities characterize electric fields and hydrogen bonding in protic, aprotic, and protein environments
J. Am. Chem. Soc., 2022, 144, 7562–7567.  https://doi.org/10.1021/jacs.2c00675


Zheng C, Mao Y, Kozuch J, Atsango A, Ji Z, Markland T, Boxer S G
A two-directional vibrational probe reveals different electric field orientations in solution and an enzyme active site
Nat. Chem., 2022, 14, 891-897.  https://doi.org/10.1038/s41557-022-00937-w


Accorsi M, Tiemann M, Wehrhan L, Finn LM, Cruz R, Rautenberg M, Emmerling F, Heberle J, Keller BG, Rademann J
Pentafluorophosphato-Phenylalanines: Amphiphilic Phosphotyrosine Mimetics Displaying Fluorine-Specific Protein Interactions
Angew Chem Int Ed Engl. 2022 Mar 18,  doi: 10.1002/anie.202203579


La Greca M,  Chen J-L, Schubert L, Kozuch J, Berneiser T,  Terpitz U,  Heberle J, Schlesinger R
The Photoreaction of the Proton-Pumping Rhodopsin 1 From the Maize Pathogenic Basidiomycete Ustilago maydis
Front Mol. Biosci. 2022, doi.org/10.3389/fmolb.2022.826990


Mous S, Gotthard G, Ehrenberg D, Sen S, Weinert T, Johnson PJM, James D, Nass K, Furrer A, Kekilli D, Ma P, Brünle S, Casadei CM, Martiel I, Dworkowski F, Gashi D, Skopintsev P, Wranik M, Knopp G, Panepucci E, Panneels V, Cirelli C, Ozerov D, Schertler GFX, Wang M, Milne C, Standfuss J, Schapiro I, Heberle J, Nogly P
Dynamics and mechanism of a light-driven chloride pump
Science. 2022 Feb 25;375(6583):845-851. doi: 10.1126/science.abj6663. Epub 2022 Feb 3


Ataka K, Drauschke J, Stulberg V, Koksch B, Heberle J
pH-induced insertion of pHLIP into a lipid bilayer: In-situ SEIRAS characterization of a folding intermediate at neutral pH
Biochim Biophys Acta Biomembr. 2022 Jan 30;1864(6):183873. doi: 10.1016/j.bbamem.2022.183873


Yaguchi M, Jia X, Schlesinger R, Jiang X, Ataka K, Heberle J
Near-Infrared Activation of Sensory Rhodopsin II Mediated by NIR-to-Blue Upconversion Nanoparticles
Front. Mol. Biosci., 2022,   https://doi.org/10.3389/fmolb.2021.782688

Kraskov A, Von Sass J, Nguyen AD,  Hoang TO, Buhrke D, Katz S, Michael N, Kozuch J , Zebger I, Siebert F, Scheerer P, Mroginski MA, Budisa N, Hildebrandt P
Local Electric Field Changes during the Photoconversion of the Bathy Phytochrome Agp2
Biochemistry, 2021, 60, 2967-2977. https://doi.org/10.1021/acs.biochem.1c00426


Carlson S, Becker M, Brünig FN, Ataka K, Cruz R, Yu L, Tang P, Kanduč M, Haag R, Heberle J, Makki H, Netz RR
Hydrophobicity of Self-Assembled Monolayers of Alkanes: Fluorination, Density, Roughness, and Lennard-Jones Cutoffs
Langmuir 2021, 37 (47):13846-13858. doi: 10.1021/acs.langmuir.1c02187


Kanevche K, Burr DJ, Nürnberg DJ, Hass PK, Elsaesser A & Heberle J
Infrared nanoscopy and tomography of intracellular structures
Communications Biology  (2021) 4: 1341. doi.org/10.1038/s42003-021-02876-7


Stripp ST
In situ infrared spectroscopy for the analysis of gas-processing metalloenzymes
ACS Catal. 2021; 11: 7845–62.  doi: 10.1021/acscatal.1c00218

Rohde M, Laun K, Zebger I, Stripp ST, Einsle O
Two ligand-binding sites in CO-reducing Vanadium Nitrogenase reveal a general mechanistic principle
Science Adv. 2021; 7: eabg4474.  doi:10.1126/sciadv.abg4474


Khushvakov J, Nussbaum R, Cadoux C, Duan J, Stripp ST, Milton RD
Untersuchung elektroenzymatischer H2‐Produktion mithilfe von Rotierende‐Ring-Scheiben‐Elektrochemie und Massenspektrometrie
Angew. Chemie 2021; 133 (18): 10089–94.  doi:  10.1002/ange.202100863


Khushvakov J, Nussbaum R, Cadoux C, Duan J, Stripp ST, Milton RD
Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry
Angew. Chemie Int. Ed. 2021; 60 (18): 10001–6.  doi: 10.26434/chemrxiv.13602869.v1


Laun K, Baranova I, Duan J, Kertess L, Wittkamp F, Apfel UP, Happe T, Senger M, Stripp ST
Site-selective protonation of the one-electron reduced cofactor in [FeFe]-hydrogenase
Dalton Trans. 2021; 5 (10): 3641–50.  doi: 10.1039/d1dt00110h


Stripp ST
Bands from Bonds
Nat. Rev. Chem. 2021; 5: 146–7.  doi: 10.1038/s41570-021-00256-7


Stripp ST, Oltmanns J, Müller CS, Ehrenberg D, Schlesinger R, Heberle J, Adrian L, Schünemann V, Pierik AJ, Soboh B.
Electron inventory of the iron-sulfur scaffold complex HypCD essential in [NiFe]-hydrogenase cofactor assembly
Biochem J (2021) 478 (17): 3281–3295. doi: 10.1042/BCJ20210224


Maia RNA, Ehrenberg D, Oldemeyer S, Knieps-Grünhagen E, Krauss U, Heberle J
Real-Time Tracking of Proton Transfer from the Reactive Cysteine to the Flavin Chromophore of a Photosensing Light Oxygen Voltage Protein
J Am Chem Soc 143(32), 12535-12542 (2021). doi: 10.1021/jacs.1c03409


Weigel A, Jacob P, Gröters D, Buberl T, Hube  M, Trubetskov M, Heberle J, Pupeza I
Ultra-rapid electro-optic sampling of octave-spanning mid-infrared waveforms
Opt. Express 29 (2021), 20747-20764; https://doi.org/10.1364/OE.423818


Baserga F, Dragelj J, Kozuch J, Mohrmann H, Knapp E-W, Stripp ST and Heberle J
Quantification of Local Electric Field Changes at the Active Site of Cytochrome c Oxidase by Fourier Transform Infrared Spectroelectrochemical Titrations
Front. Chem. 9:669452 (2021); doi: 10.3389/fchem.2021.669452


Mueller NS, Pfitzner E, Okamura Y, Gordeev G, Kusch P, Lange H, Heberle J, Schulz F, Reich S
Surface-Enhanced Raman Scattering and Surface-Enhanced Infrared Absorption by Plasmon Polaritons in Three-Dimensional Nanoparticle Supercrystals

ACS Nano 15, 5523-5533 (2021); https://pubs.acs.org/doi/10.1021/acsnano.1c00352


Kruse F, Nguyen, A.D, Dragelj J, Heberle J, Hildebrandt P,  Mroginski MA, Weidinger IM
A Resonance Raman Marker Band Characterizes the Slow and Fast Form of Cytochrome c Oxidase
J Am Chem Soc. 143, 2769-2776 (2021); doi: 10.1021/jacs.0c10767


Bülbül, E., Hegemann, D., Ataka, K., Lehner, S., Heberle, J., Heuberger, M.
Confined hydration in nanometer-graded plasma polymer films: Insights from surface-enhanced infrared absorption spectroscopy
Surf. Interfaces 23, 100922 /2021); doi.org/10.1016/j.surfin.2020.100922


Hulin, T., Hirota, S., Stripp, S.T.
Proton Transfer Mechanisms in Bimetallic Hydrogenases
Acc. Chem. Res. 54 (1), 232-241 (2021); https://pubs.acs.org/doi/10.1021/acs.accounts.0c00651


Abul-Futouh, H., Almazahreh, L. R., Abaalkhail, S. J., Görls, H., Stripp, S. T., Weigand, W.
Ligand effects on structural, protophilic and reductive features of stannylated dinuclear iron dithiolato complexes 
New. J. Chem. 45, 36-44 (2021);  https://doi.org/10.1039/D0NJ04790B


Stripp, S. T., Mebs, S., Haumann, M.
Temperature Dependence of Structural Dynamics at the Catalytic Cofactor of [FeFe]- hydrogenase
Inorg. Chem. 59 (22): 16474 – 88 (2020); doi.org/10.1021/acs.inorgchem.0c02316


Land, H., Sekretaryova, A. L., Huang, P., Redman, H. J., Németh, B., Polidori, N., Mészáros, L., Senger, M., Stripp, S. T., Berggren, G.
Characterization of a putative sensory [FeFe]-hydrogenase provides new insight into the role of the active site architecture
Chem. Sci. 2020; https://doi.org/10.1039/D0SC03319G


Németh, B., Senger, M., Redman, H. J., Ceccaldi, P., Broderick, J., Magnuson, A., Stripp, S. T., Haumann, M., Berggren, G.
[FeFe]-Hydrogenase Maturation: H-Cluster Assembly Intermediates Tracked by Electron Paramagnetic Resonance, Infrared, and X-Ray Absorption Spectroscopy
J. Biol. Inorg. Chem. 25: 777 – 88 (2020); doi.org/10.1007/s00775-020-01799-8


Land, H., Senger, M., Berggren G., Stripp, S. T.
Current State of [FeFe]-Hydrogenase Research: Biodiversity and Spectroscopic Investigations 
ACS Catal. 10 (13): 7069 – 86 (2020); doi.org/10.1021/acscatal.0c01614


Ulrich, G., P!tzner, E. Hoehl, A. Liao, J-W., Zadvorna, O., Schweicher, G., Sirringhaus, H., Heberle, J., Kästner, B., Wunderlich, J.,  and Venkateshvaran, D.
Thermoelectric nanospectroscopy for the imaging of molecular fingerprints
Nanophotonics 9,  4347–4354 (2020); doi.org/10.1515/nanoph-2020-0316


Janda, T., Godinho, J., Ostatnicky, T., Pfitzner, E.,  Ulrich, G., Hoehl, A., Reimers, S., Šobáˇn, Z., Metzger, T., Reichlová, H., Novák, V.,  Campion, R. P.,  Heberle, J., Wadley, P.,   Edmonds, K. W.,  Amin, O. J., Chauhan, J. S.,   Dhesi, S. S., Maccherozzi, F.,  Otxoa, R. M., Roy, P. E.,  Olejník, K.,  Nˇemec, P.,  Jungwirth, T.,  Kaestner, B., and Wunderlich, J.
Magneto-Seebeck microscopy of domain switching in collinear antiferromagnet CuMnAs
Phys. Rev.  Mat 4, 094413 (2020); doi.org/10.1103/PhysRevMaterials.4.094413


Hugentobler, K. G., Heinrich, D., Berg, J., Heberle, J., Brzezinski, P., Schlesinger, R. and Block, S.
Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome c Oxidase
Int. J. Mol. Sci., 21(19), 6981 (2020); doi.org/10.3390/ijms21196981


Pfitzner E, Heberle J.
Infrared Scattering-Type Scanning Near-Field Optical Microscopy of Biomembranes in Water.
J. Phys. Chem. Lett. 11, 8183−8188 (2020), doi: 10.1021/acs.jpclett.0c01769


Skopintsev, P., Ehrenberg, D., Weinert, T., James, D., Kar, R.K., Johnson, P.J.M., Ozerov, D., Furrer, A., Mous, S., Martiel, I., Dworkowski, F., Nass, K., Knopp, G., Cirelli,C., Gashi, D., Wranik, M., Gruhl, T., Kekilli, D., Brünle, S., Deupi, X., Schertler, G., Benoit, R., Panneels, V., Nogly, P., Schapiro, I., Milne,C., Heberle, J., Standfuss, J.
Femtosecond to millisecond structural changes in a light-driven sodium pump
Nature 583, 314–318 (2020), doi: 10.1038/s41586-020-2307-8


Harris A., Lazaratos M., Siemers M., Watt E., Hoang A., Tomida S., Schubert L., Saita M., Heberle J., Furutani Y., Kandori H., Bondar A.N., Brown L.S.
Mechanism of Inward Proton Transport in an Antarctic Microbial Rhodopsin
J Phys Chem B 124 (24), 4851-4872 (2020),  doi: 10.1021/acs.jpcb.0c02767


Mészáros, L.S., Ceccaldi, P., Lorenzi, M., Redman, H.J., Pfitzner, E., Heberle, J., Senger, M., Stripp, S.T., Berggren, G.
Spectroscopic investigations under whole-cell conditions provide new insight into the metal hydride chemistry of [FeFe]-hydrogenase
Chem. Sci. 11, 4608-4617 (2020), doi: 10.1039/D0SC00512F


Kruse F., Nguyen A.D., Dragelj J., Schlesinger R., Heberle J., Mroginski M.A., Weidinger I.M.
Characterisation of the Cyanate Inhibited State of Cytochrome c Oxidase
Sci Rep. Mar 2;10(1):3863 (2020). doi: 10.1038/s41598-020-60801-0.


Huang, G., Wagner, T., Wodrich, M.D., Ataka, K., Bill, E., Ermler, U., Hu, X., Shima, S.
The atomic-resolution crystal structure of activated [Fe]-hydrogenase
Nature Catalysis 2, 537-543 (2019)


Ehrenberg, D., Krause, N., Saita, M., Bamann, C., Kar, R.K., Hoffmann, K.,  Heinrich, D., Schapiro, I., Heberle, J., and Schlesinger, R.
Atomistic insight into the role of threonine 127 in the functional mechanism of channelrhodopsin-2
Appl. Sci. 2019, 9(22), 4905; https://doi.org/10.3390/app9224905


Kopyshev, A., Kanevche, K., Lomadze, N., Pfitzner, E., Loebner, S., Patil, R.R., Genzer, J., Heberle, J., Santer, S.
Light-Induced Structuring of Photosensitive Polymer Brushes
ACS Appl. Polym. Mater. 2019, 1, 11, 3017-3026


Senger, M., Eichmann, V., Laun, K., Duan, J., Wittkamp, F., Knör, G., Apfel, U.-P., Happe, T., Winkler, M., Heberle, J., Stripp, S. T.
How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer
J. Am. Chem. Soc. (2019), doi.org/10.1021/jacs.9b09225


Land, H., Ceccaldi, P., Mészáros, L. S., Lorenzi, M.,  Redman, H. J., Senger, M., Stripp, S. T., Berggren, G.,
Discovery of novel [FeFe]-hydrogenases for biocatalytic H2-production
Chem. Sci., (2019), Advance Article, doi 10.1039/C9SC03717A


Duan, J., Mebs, S., Laun, K., Wittkamp, F., Heberle, J., Happe, T., Hofmann, E., Apfel, U.-P., Winkler, M., Senger, M., Haumann, M., Stripp, S.T.
Geometry of the Catalytic Active Site in [FeFe]-Hydrogenase Is Determined by Hydrogen Bonding and Proton Transfer
ACS Catal. (2019), 9, 10, 9140-9149. doi.org/10.1021/acscatal.9b02203

Nass Kovacs, G., Colletier, J.-P. , Grünbein, M. L., Yang, Y., Stensitzki, T. , Batyuk, A., Carbajo, S., Doak, R. B., Ehrenberg, D., Foucar, L., Gasper, R., Gorel, A.,  Hilpert, M., Kloos, M., Koglin, J. E., Reinstein, J., Roome, C. M., Schlesinger, R., Seaberg, M., Shoeman, R. L.,  Stricker, M., Boutet, S., Haacke, S.,  Heberle, J., Heyne, K., Domratcheva, T., Barends, T. R. M., and Schlichting, I.
Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
Nat. Commun. 10(1): 3177 (2019). doi 10.1038/s41467-019-10758-0


Pan, H.J., Huang, G., Wodrich, M.D., Tirani, F.F., Ataka, K., Shima, S., Hu, X.
A catalytically active [Mn]-hydrogenase incorporating a non-native metal cofactor
Nat Chem. (2019) Jul;11(7):669-675. doi: 10.1038/s41557-019-0266-1


Watanabe, T.,  Wagner, T.,  Huang, G.,  Kahnt, J., Ataka, K., Ermler, U., Shima, S.
The Bacterial [Fe]-Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates
Angew. Chem. 58, 3506-3510 (2019), doi: https://doi.org/10.1002/anie.201813465


Mebs, S., Duan, J., Wittkamp, F., Stripp, S. T., Happe, T., Apfel, U.-P., Winkler, M., and Haumann, M.
Differential Protonation at the Catalytic Six-Iron Cofactor of [FeFe]-Hydrogenases Revealed by 57Fe Nuclear Resonance X-ray Scattering and Quantum Mechanics/Molecular Mechanics Analyses

Inorg. Chem. (2019), DOI: 10.1021/acs.inorgchem.9b00100


Ehrenberg, D., Varma, N., Deupi, X., Koyanagi, M., Terakita, A., Schertler, G.F.X., Heberle, J., Lesca, E.
The two-photon reversible reaction of the bistable jumping spider rhodopsin-1
Biophysical Journal 116, 1248–1258 (2019), doi: https://doi.org/10.1016/j.bpj.2019.02.025


Huang, GF; Wagner, T; Ermler; Bill, E; Ataka, K; Shima, S.
Dioxygen Sensitivity of [Fe]-Hydrogenase in the Presence of Reducing Substrates
Angew. Chem. 57, 4917-4920,  (2018), doi: https://doi.org/10.1002/anie.201712293


Pfitzner, E., Hu, X., Schumacher, H. W., Hoehl, A., Venkateshvaran, D., Cubukcu, M., Liao, J.-W., Auffret, S.,  Heberle, J., Wunderlich, J., and Kästner, B.
Near-field magneto-caloritronic nanoscopy on ferromagnetic nanostructures
AIP
AIP Advances  8, 125329 (2018)


Hua, H., Ataka, K., Mennyd, A., Fouratia, Z., Saugueta, L., Corringer, P.J., Koehl, P., Heberle, J., and Delarue, M.
Electrostatics, proton sensor, and networks governing the gating transition in GLIC, a proton-gated pentameric ion channel

Proc. Acad. Natl. Sci. USA 115, E12172-E12181 (2018)


Duan, J., Senger, M., Esselborn, J., Engelbrecht, V., Wittkamp, F., Apfel, U.-P., Hofmann, E., Stripp, S. T.,  Happe, T. & Winkler, M.
Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases
Nature Commun. 9, 4726 (2018), doi: 10.1038/s41467-018-07140-x


Senger, M., Laun, K., Soboh, B. and Stripp, S. T.
Infrared Characterization of the Bidirectional Oxygen-Sensitive [NiFe]-Hydrogenase from E. coli
Catalysts 2018, 8, 530; doi:10.3390/catal8110530


Saita, M., Pranga-Sellnau F., Resler T., Schlesinger R., Heberle J., Lórenz-Fonfría, V.A.
Photoexcitation of the P4480 state induces a secondary photocycle that potentially desensitizes channelrhodopsin-2.
J Am Chem Soc. 140, 9899-9903 (2018); doi: 10.1021/jacs.8b03931


Haumann, M., Stripp Sven T.
The Molecular Proceedings of Biological Hydrogen Turnover
Acc. Chem. Res 51, 1755–1763 (2018), 10.1021/acs.accounts.8b00109


Laun, K., Mebs, S.,  Duan, J., Wittkamp, F.,  Apfel, U.-P., Happe, T.,  Winkler, M., Haumann, M., Stripp, S. T.
Spectroscopical investigations on the redox chemistry of [FeFe]-hydrogenase in the presence of carbon monoxide. Molecules, accepted (2018)
Molecules 23(7), 1669 (2018); https://doi.org/10.3390/molecules23071669


Apfel, U.-P. und Stripp, S. T.
Wasserstoffproduktion nach dem Vorbild der Natur 
GIT-Labor (2018), 6, 28-31 https://www.git-labor.de/forschung/life-sciences-biotechnologie/wasserstoffproduktion-nach-dem-vorbild-der-natur


Wittkamp, F., Senger, M., Stripp, S. T. und Apfel, U.-P.
[FeFe]-Hydrogenases: recent developments and future perspectives
Chem. Commun. 54, 5934 (2018),  doi: 10.1039/C8CC01275J


Pfitzner, E., Seki, H., Schlesinger, R., Ataka, K., Heberle, J.
Disc Antenna Enhanced Infrared Spectroscopy: From Self-Assembled Monolayers to Membrane Proteins
ACS Sens. 3, 5, 984-991 (2018), doi: 10.1021/acssensors.8b00139


Harris, A., Saita, M., Resler, T.,  Hughes-Visentin, A., Maia, R.,  Pranga-Sellnau, F., Bondar, A.-N., Heberle, J., Brown, L. S.
Molecular details of the unique mechanism of chloride transport by a cyanobacterial rhodopsin
Phys. Chem. Chem. Phys. (2018), 20, 3184-3199, DOI: 10.1039/c7cp06068h


Mebs, S., Kositzki, R., Duan, J., Senger, M., Wittkamp, F., Apfel, U.-P., Happe, T., Stripp, S.T., Winkler, M., Haumann, M.
Hydrogen and oxygen trapping at the H-cluster of [FeFe]‑hydrogenase revealed by site-selective spectroscopy and QM/MM calculations
Biochim. Biophys. Acta 1859, 28-41 (2018),  DOI: 10.1016/j.bbabio.2017.09.003


Senger, M., Mebs, S.,  Duan, J., Shulenina, O., Laun, K., Kertess, L., Wittkamp, F., Apfel, U.-P., Happe, T., Winkler, M., Haumann, M., and Stripp, S.T.
Protonation/reduction dynamics at the [4Fe–4S] cluster of the hydrogen-forming cofactor in [FeFe]-hydrogenases
Phys. Chem. Chem. Phys. (2018), 20, 3128-3140,  DOI: 10.1039/C7CP04757F   


Daldrop, J.O., Saita, M., Heyden, M., Lorenz-Fonfria, V. A., Heberle. J. & Netz, R. R.
Orientation of non-spherical protonated water clusters revealed by infrared absorption dichroism
Nature Commun. 9, 311 (2018), DOI: 10.1038/s41467-017-02669-9


Schultz, B.-J., Mohrmann, H., Lórenz-Fonfría, V.A., Heberle J.
Protein dynamics observed by tunable mid-IR quantum cascade lasers across the time range from 10 ns to 1 s
Spectrochim. Acta A (2018), 188, 666-674,  http://dx.doi.org/10.1016/j.saa.2017.01.010



Adamson H, Robinson M, Bond PS, Soboh B, Gillow K, Simonov AN, Elton DM, Bond AM, Sawers RG, Gavaghan DJ, Parkin A.
Analysis of HypD Disulfide Redox Chemistry via Optimization of Fourier Transformed ac Voltammetric Data
Anal Chem. 2017 Feb 7;89(3):1565-1573. doi: 10.1021/acs.analchem.6b03589. Epub 2017 Jan 19


Cottin, H., Kotler, J.M., Billi, D., Cockell, C., Demets, R., Ehrenfreund, P., Elsaesser, A., d’Hendecourt, L., Van Loon, J.J., Martins, Z. and Onofri, S.
Space as a tool for astrobiology: review and recommendations for experimentations in Earth orbit and beyond
Space Science Reviews, 209(1-4), pp.83-181. (2017), DOI: 10.1007/s11214-017-0365-5


Martins, Z., Cottin, H., Kotler, J.M., Carrasco, N., Cockell, C.S., de la Torre Noetzel, R., Demets, R., De Vera, J.P., d’Hendecourt, L., Ehrenfreund, P. and Elsaesser, A.
Earth as a Tool for Astrobiology—A European Perspective
Space Science Reviews, 209(1-4), pp.43-81. (2017), DOI: 10.1007/s11214-017-0369-1


Lorenz-Fonfria VA, Saita M, Lazarova T, Schlesinger R, Heberle J.
pH-sensitive vibrational probe reveals a cytoplasmic protonated cluster in bacteriorhodopsin
Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E10909-E10918. doi: 10.1073/pnas.1707993114. Epub 2017 Dec 4


Harris, A., Saita, M., Resler, T.,  Hughes-Visentin, A., Maia, R.,  Pranga-Sellnau, F., Bondar, A.-N., Heberle, J., Brown, L. S.
Molecular details of the unique mechanism of chloride transport by a cyanobacterial rhodopsin
Phys. Chem. Chem. Phys. 2017, DOI: 10.1039/c7cp06068h


Senger, M., Laun, K., Wittkamp, F., Duan, J., Haumann, M., Happe, T., Winkler, M., Apfel, U.-P. and Stripp, S. T.
Protonengekoppelte Reduktion des katalytischen [4Fe-4S]-Zentrums in [FeFe]-Hydrogenasen
Angew. Chem.. (2017), doi:10.1002/ange.201709910


Senger, M., Laun, K., Wittkamp, F., Duan, J., Haumann, M., Happe, T., Winkler, M., Apfel, U.-P., Stripp, S.T.
Proton-Coupled Reduction of the Catalytic [4Fe-4S] Cluster in [FeFe]-Hydrogenases

Angew. Chem. Int. Ed.. 
(2017), doi:10.1002/anie.201709910

Mebs, S., Senger, M., Duan, J., Wittkamp, F., Apfel, U.-P., Happe, T., Winkler, M., Stripp, S.T., and Haumann, M.
Bridging Hydride at Reduced H-Cluster Species in [FeFe]-Hydrogenases
Revealed by Infrared Spectroscopy, Isotope Editing, and Quantum Chemistry

J. Am. Chem. Soc., Just Accepted Manuscript. doi: 10.1021/jacs.7b07548, Publication Date (Web): August 21, 2017


Berntsson O, Diensthuber RP, Panman MR, Björling A, Gustavsson E, Hoernke M, Hughes AJ, Henry L, Niebling S, Takala H, Ihalainen JA, Newby G, Kerruth S, Heberle J, Liebi M, Menzel A, Henning R, Kosheleva I, Möglich A, Westenhoff S.
Sequential conformational transitions and α-helical supercoiling regulate a sensor histidine kinase
Nat Commun. 2017 Aug 18;8(1):284. doi: 10.1038/s41467-017-00300-5


Harris NJ, Reading E, Ataka K, Grzegorzewski L, Charalambous K, Liu X, Schlesinger R, Heberle J, Booth PJ.
Structure formation during translocon-unassisted co-translational membrane protein folding
Sci Rep. 2017 Aug 14;7(1):8021. doi: 10.1038/s41598-017-08522-9


Mohrmann H, Heberle J.
Reply to "Comment on 'Transient Conformational Changes of Sensory Rhodopsin II Investigated by Vibrational Stark Effect Probes'"
J Phys Chem B. 2017 Aug 3;121(30):7397-7399. doi: 10.1021/acs.jpcb.7b03453. Epub 2017 Jul 24


Winkler, M., Senger, M., Duan, J., Esselborn, J., Wittkamp, F., Hofmann, E., Apfel, U.-P., Stripp, S.T., Happe,
Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases
Nature Communications 8 (2017); doi: 10.1038/ncomms16115


Senger, M., Stripp, S.T., Soboh, B.
Proteolytic Cleavage Orchestrates Cofactor Insertion and Protein Assembly in [NiFe]-hydrogenase Biosynthesis
J. Biol Chem. (2017); http://www.jbc.org/cgi/doi/10.1074/jbc.M117.788125

Senger, M., Stripp, S.T.
Wasserstoffkatalyse in Mikroalgen
Nachrichten aus der Chemie, (2017) 65, 123-127;  doi: 10.1002/nadc.20174056774


Kovácsová, G., Grünbein, M.L., Kloos, M., Barends, T.R.M., Schlesinger, R., Heberle, J., Kabsch, W., Shoeman, R.L., Doaka, R.B., and Schlichting, I.
Viscous hydrophilic injection matrices for serial crystallography
IUCrJ (2017) 4, doi:10.1107/S2052252517005140


Kerruth, S., Langner, P., Raffelberg, S., Gärtner, W., Heberle, J.
Characterization of the blue-light activated adenylyl cyclase mPAC by flash photolysis and FTIR spectroscopy
Photochem. Photobiol. (2017) 93, 637–907; 10.1111/php.12746


Kottke, T., Lórenz-Fonfría V.A., Heberle, J.
The Grateful Infrared – Sequential Protein Structural Changes Resolved by IR Difference Spectroscopy
J. Phys. Chem. B (2017) 121, 335-350; doi:10.1021/acs.jpcb.6b09222

Hoersch, D., Kortemme T.
A model for the molecular mechanism of an engineered light-driven protein machine
Structure. 24(4):576-584 (2016)

Hoersch, D.
Engineering a light-controlled F1 ATPase using structure-based protein design
Peer J. 4:e2286 (2016)

Schreiner, M., Schlesinger, R., Heberle, J. & Niemann, H.H.
Crystal structure of Halobacterium salinarum halorhodopsin with a partially depopulated primary chloride-binding site
Acta Cryst. (2016). F72, 692-699, doi:10.1107/S2053230X16012796

Senger, M., Mebs, S., Duanc, J., Wittkamp, F., Apfel, U.-P., Heberle, J., Haumann, M., and Stripp, S.T. 

Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics
Proc. Natl. Acad. Sci USA, 113, 8454-8459 (2016), doi:10.1073/pnas.1606178113

Süss, B., Ringleb, F.  and Heberle J.
New Ultrarapid-Scanning Interferometer for FT-IR spectroscopy with Microsecond Time-resolution
Rev. Sci. Instr. 87, 063113 (2016); doi: 10.1063/1.4953658

Stensitzki T, Yang Y, Muders V, Schlesinger R, Heberle J, Heyne K.
Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization
Struct. Dyn.3, 043208 (2016); http://dx.doi.org/10.1063/1.4948338

Mohrmann H, Kube I, Lórenz-Fonfría VA, Engelhard M, Heberle J.
Transient Conformational Changes of Sensory Rhodopsin II Investigated by Vibrational Stark Effect Probes
J Phys Chem B (2016) 120, 4383–4387; doi: 10.1021/acs.jpcb.6b01900

Schnedermann C, Muders V, Ehrenberg D, Schlesinger R, Kukura P, Heberle J.
Vibronic Dynamics of the Ultrafast all-trans to 13-cis Photoisomerization of Retinal in Channelrhodopsin-1.
J Am Chem Soc. 2016 Apr 13;138(14):4757-62. doi: 10.1021/jacs.5b12251

Baumann A, Kerruth S, Fitter J, Büldt G, Heberle J, Schlesinger R, Ataka K.
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression.
PLoS One. 2016 Mar 15;11(3):e0151051. doi: 10.1371/journal.pone.0151051

B. Green, S. Kovalev, V. Asgekar, G. Geloni, U. Lehnert, T. Golz, M. Kuntzsch, C. Bauer, J. Hauser, J. Voigtlaender, B. Wustmann, I. Koesterke, M. Schwarz, M. Freitag, A. Arnold, J. Teichert, M. Justus, W. Seidel, C. Ilgner, N. Awari, D. Nicoletti, S. Kaiser, Y. Laplace, S. Rajasekaran, L. Zhang, S. Winnerl, H. Schneider, G. Schay, I. Lorincz, A. A. Rauscher, I. Radu, S. Mährlein, T. H. Kim, J. S. Lee, T. Kampfrath, S. Wall, J. Heberle, A. Malnasi-Csizmadia, A. Steiger, A. S. Müller, M. Helm, U. Schramm, T. Cowan, P. Michel, A. Cavalleri, A. S. Fisher, N. Stojanovic, M. Gensch
High-Field High-Repetition-Rate Sources for the Coherent THz Control of Matter
Scientific Reports 6, Article number: 22256 (2016) doi:10.1038/srep22256

Heberle, J.
Time-Resolved and Surface-Enhanced Infrared Spectroscopy,
in “Pumps, Channels and Transporters”, (Ronald J. Clarke & Mohammed A.A. Khalid, Eds.),
pp. 245-265, John Wiley & Sons, New Jersey (2015)
ISBN 978-1-118-85880-6


Lórenz-Fonfría VA, Bamann C, Resler T, Schlesinger R, Bamberg E, Heberle J
Temporal evolution of helix hydration in a light-gated ion channel correlates with ion conductance.
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5796-804. doi: 10.1073/pnas.1511462112. Epub 2015 Oct 12


Shima S, Chen D, Xu T, Wodrich MD, Fujishiro T, Schultz KM, Kahnt J, Ataka K, Hu X
Reconstitution of [Fe]-hydrogenase using model complexes
Nat Chem. 2015 Dec;7(12):995-1002. doi: 10.1038/nchem.2382

Hidese R, Ataka K, Bill E, Shima S
CuI and H2 O2 Inactivate and FeII Inhibits [Fe]-Hydrogenase at Very Low Concentrations
Chembiochem. 16(13):1861-1865 (2015). doi: 10.1002/cbic.201500318. [Epub ahead of print]


Fujishiro T, Ataka K, Ermler U, Shima S
Towards a functional identification of catalytically inactive [Fe]-hydrogenase paralogs
FEBS J. 2015 Sep;282(17):3412-23. doi: 10.1111/febs.13351. Epub 2015 Jul 14


Fuerst O, Lin Y, Granell M, Leblanc G, Padrós E, Lórenz-Fonfría VA, Cladera J
The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES
J Biol Chem. 2015 Jun 26;290(26):16261-71. doi: 10.1074/jbc.M115.642678


Stripp ST, Lindenstrauss U, Sawers RG, Soboh B
Identification of an Isothiocyanate on the HypEF Complex Suggests a Route for Efficient Cyanyl-Group Channeling during [NiFe]-Hydrogenase Cofactor Generation
PLoS One. 2015 Jul 17;10(7):e0133118. doi: 10.1371/journal.pone.0133118


Stensitzki T, Muders V, Schlesinger R, Heberle J, Heyne K
The primary photoreaction of channelrhodopsin-1: wavelength dependent photoreactions induced by ground-state heterogeneity
Front Mol Biosci. 2015 Jul 22;2:41. doi: 10.3389/fmolb.2015.00041. eCollection 2015


Sezer M, Kielb P, Kuhlmann U, Mohrmann H, Schulz C, Heinrich D, Schlesinger R, Heberle J, Weidinger IM,
Surface Enhanced Resonance Raman Spectroscopy Reveals Potential Induced Redox and Conformational Changes of Cytochrome c Oxidase on Electrodes
J Phys Chem B. 2015 Jul 30;119(30):9586-9591.


Kuschewski F, Kehr SC, Green B, Bauer Ch, Gensch M, Eng LM,
Optical nanoscopy of transient states in condensed matter
Sci Rep. 2015 Jul 28;5:12582. doi: 10.1038/srep12582


Resler T, Schultz BJ, Lórenz-Fonfría VA, Schlesinger R, Heberle J
Kinetic and Vibrational Isotope Effects of Proton Transfer Reactions in Channelrhodopsin-2
Biophys J. 2015 Jul 21;109(2):287-97. doi: 10.1016/j.bpj.2015.06.023.


Schreiner, M., Schlesinger, R., Heberle, J., Niemann, H.H.
Structure of Halorhodopsin from Halobacterium salinarum in a new crystal form that imposes little restraint on the E-F loop
J Struct Biol. 2015 Jun;190(3):373-8. doi: 10.1016/j.jsb.2015.04.010. Epub 2015 Apr 24.


Stripp, S.T. and Heberle J
Photosynthesis and hydrogen metabolism revisited. On the potential of light-driven hydrogen production in vitro
in “Biohydrogen”, (Matthias Rögner, Ed.)
pp. 211-229, Walter de Gruyter GmbH, Berlin (2015); ISBN 978-3-11-033645-0


Lórenz-Fonfría, V.A., Schultz, B.J., Resler, T., Schlesinger, R., Bamann, C., Bamberg, E., Heberle, J.
Pre-Gating Conformational Changes in the ChETA Variant of Channelrhodopsin-2 Monitored by Nanosecond IR Spectroscopy
J. Am. Chem. Soc. 137, 1850−1861 (2015); doi: 10.1021/ja5108595

Lórenz-Fonfría, V A, Muders, V, Schlesinger, R, Heberle, J
Changes in the hydrogen-bonding strength of internal water molecules and cysteine residues in the conductive state of channelrhodopsin-1
J. Chem. Phys. 141 (22) (2014); doi:10.1063/1.4895796


Stripp S T, Lindenstrauss U, Granich C, Sawers R G, Soboh B
The Influence of Oxygen on [NiFe]–HydrogenaseCofactor Biosynthesis and How Ligation of CarbonMonoxide Precedes Cyanation
PLoS ONE 9(9): e107488. doi:10.1371/journal.pone.0107488 (2014)

Soboh B, Lindenstrauss U, Granich C, Javed M, Herzberg M, Thomas C, Stripp S T
[NiFe]-hydrogenase maturation in vitro: analysis of the roles of the HybG and HypD accessory proteins
Biochem. J. (2014) Immediate Publication, doi:10.1042/BJ20140485 (2014)

Kerruth S, Ataka K, Frey D, Schlichting I, Heberle J
Aureochrome 1 Illuminated: Structural Changes of a Transcription Factor Probed by MolecularSpectroscopy
PLoS ONE 9(7): e103307. doi:10.1371/journal.pone.0103307 (2014)

Lórenz-Fonfría, V. A., Heberle, J.
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
J. Vis. Exp. (88), e51622, doi:10.3791/51622 (2014)

Muders V, Kerruth S, Lórenz-Fonfría VA, Bamann C, Heberle J, Schlesinger R.
Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1
FEBS Lett. 588,  2301–2306 (2014)


Rüther A, Pfeifer M, Lórenz-Fonfría VA, Lüdeke S.
pH titration monitored by quantum cascade laser-based vibrational circular dichroism
J Phys Chem B. 118, 3941-9 (2014). doi: 10.1021/jp4122886. Epub 2014 Apr 2.


Rüther A, Pfeifer M, Lórenz-Fonfría VA, Lüdeke S.
Reaction Monitoring Using Mid-Infrared Laser-Based Vibrational Circular Dichroism
Chirality 26, 490-6 (2014). doi: 10.1002/chir.22307


Heberle J, Deupi X, Schertler G
Retinal proteins - You can teach an old dog new tricks
Biochim Biophys Acta. 1837, 531-2 (2014)

Lorenz-Fonfria, V A, Heberle, J
Channelrhodopsin unchained: Structure and mechanism of a light-gated cation channel
Biochim Biophys Acta. 1837, 626-642 (2014)

Munter LM, Sieg H, Bethge T, Liebsch F, Bierkandt FS, Schleeger M, Bittner HJ, Heberle J, Jakubowski N, Hildebrand PW, Multhaup G
Model peptides uncover the role of the β-secretase transmembrane sequence in metal-ion mediated oligomerization
J Am Chem Soc. 135, 19354-61 (2013)

Amenabar I, Poly S, Nuansing W, Hubrich EH, Govyadinov A, Huth F, Krutokhvostov R, Zhang L, Knez M, Heberle J, Bittner A, Hillenbrand, R
Structural analysis and mapping of individual protein complexes by infrared nanospectroscopy
Nat Commun. 2013 Dec 4;4:2890. doi: 10.1038/ncomms3890.  (2013)

Lin Y, Fuerst O, Granell M, Leblanc G, Lórenz-Fonfría V, Padrós E
The substitution of Arg149 with Cys fixes the melibiose transporter in an inward-open conformation.
Biochim Biophys Acta. 1828,1690-9 (2013)

Woelke AL, Galstyan G, Galstyan A, Meyer T, Heberle J, Knapp EW
Exploring the Possible Role of Glu286 in CcO by Electrostatic Energy Computations Combined with Molecular Dynamics
J Phys Chem B. 117, 12432-41 (2013)

Krause N, Engelhard C, Heberle J, Schlesinger R, Bittl R
Structural differences between the closed and open states of channelrhodopsin-2 as observed by EPR spectroscopy
FEBS Lett. 587, 3309-3313 (2013)

Soboh B, Stripp ST, Bielak C, Lindenstrauß U, Braussemann M, Javaid M, Hallensleben M, Granich C, Herzberg M, Heberle J, Gary Sawers R
The [NiFe]-hydrogenase accessory chaperones HypC and HybG of Escherichia coli are iron- and carbon dioxide-binding proteins
FEBS Lett. 587, 2512-6 (2013)

Pfitzner E, Wachauf C, Kilchherr F, Pelz B, Shih WM, Rief M, Dietz H
Rigid DNA Beams for High-Resolution Single-Molecule Mechanics
Angew. Chem. Int. Ed. 52, 1–7 (2013)

Hatting B, Heeg S, Ataka K, Heberle J, Hennrich F, Kappes MM, Krupke R, and Reich S
Fermi energy shift in deposited metallic nanotubes: A Raman scattering study
Phys. Rev. B 87, 165442/1-5 (2013)

Ataka K, Stripp S, and Heberle J
Surface-enhanced infrared absorption spectroscopy (SEIRAS) to probe monolayers of membrane proteins
Biochim. Biophys. Acta 1828, 2283-93 (2013)

Stripp ST, Soboh B, Lindenstrauß U, Braussemann M, Herzberg M, Nies DH, Sawers RG, Heberle J 
HypD is the Scaffold Protein for Fe-(CN)2CO Cofactor Assembly in [NiFe]-Hydrogenase Maturation
Biochemistry 52, 3289-96 (2013)

Neumann-Verhoefen MK, Neumann K, Bamann C, Radu I, Heberle J, Bamberg E, Wachtveitl JL
Ultrafast infrared spectroscopy on channelrhodopsin-2 reveals efficient energy transfer from the retinal chromophore to the protein
J Am Chem Soc. 135, 6968-76 (2013)

Lórenz-Fonfría, V.A., Resler, T., Krause, N., Nack, M., Gossing, M., Fischer von Mollard, G., Bamann, C., Bamberg, E., Schlesinger, R., and Heberle, J.
Transient protonation changes in channelrhodopsin-2 and their relevance to channel gating
Proc. Natl. Acad. Sci USA 110 (14), E1273-E1281 (2013)

Ataka K and Heberle J,
Surface Enhanced Infrared Absorption Spectroscopy
in “Encyclopedia of Biophysics”, (Gordon C.K. Roberts (Ed.) and European Biophysical Societies Association)
pp. 2528-2531, Springer, Berlin (2013)
ISBN 978-3-642-16711-9

Soboh B, Stripp ST, Muhr E, Granich C, Braussemann M, Herzberg M, Heberle J, Gary Sawers R
[NiFe]-hydrogenase maturation: Isolation of a HypC-HypD complex carrying diatomic CO and CN(-) ligands
FEBS Lett. 586, 3882-7 (2012)

Schick M, Xie XL, Ataka K, Kahnt J, Linne U, Shima S
Biosynthesis of the Iron-Guanylylpyridinol Cofactor of [Fe]-Hydrogenase in Methanogenic Archaea as Elucidated by Stable-Isotope Labeling
J Am Chem Soc 134, 3271-3280 (2012)

Shima S, Schick M; Kahnt J, Ataka K, Steinbach K, Linne U
Evidence for acyl-iron ligation in the active site of [Fe]-hydrogenase provided by mass spectrometry and infrared spectroscopy
Dalton Trans. 41, 767-771 (2012)

Burney S, Wenzel R, Kottke T, Roussel T, Hoang N, Bouly J-P, Bittl R, Heberle J, and Ahmad M 
Single amino acid substitution reveals latent photolyase activity in Arabidopsis cry1
Angew. Chem. 124, 9490-9494 (2012)

Schleeger M, Heberle J, and Kakorin S
Simplifying the analysis of enzyme kinetics of cytochrome C oxidase by the Lambert-W function
Open J. Biophys. 2, 117-129 (2012)

Nack M, Radu I, Schultz B-J, Resler T, Schlesinger R, Bondar A-N, del Val C, Abbruzzetti S, Viappiani C, Bamann C, Bamberg E, and Heberle J
Kinetics of Proton Release and Uptake by Channelrhodopsin-2
FEBS Lett. 586, 1344-8 (2012)

Kirchberg K, Kim TY, Möller M, Skegro D, Dasara Raju G, Granzin J, Büldt G, Schlesinger R, Alexiev U
Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process
Proc. Natl. Acad Sci USA 108, 18690-5 (2011) 

Ballout F, Krassen H, Kopf I, Ataka K, Bründermann E, Heberle J, Havenith M
Scanning near-field IR microscopy of proteins in lipid bilayers
Phys. Chem. Chem. Phys. 13, 21432-21436 (2011) 

Schleeger M, Radu I, Heberle J
Time-resolved FT-IR Spectroscopy for the Elucidation of Protein Function’,
in: "Supramoleclar Structure and Function 10",
Eds. Brnjas-Kraljevic, J., Pifat-Mrzljak, G., Springer, pp. 31-40 (2011)

Krassen H, Stripp ST, Böhm N, Berkessel A, Happe T, Ataka K, and Heberle J
Tailor-Made Modification of a Gold Surface for the Chemical Binding of a High-Activity [FeFe] Hydrogenase
Eur. J. Inorg. Chem. 2011, 1138–1146 (2011)

Shima S, Ataka K
Isocyanides inhibit [Fe]-hydrogenase with very high affinity
FEBS Lett. 585, 353-356 (2011)

Bauer C, Rabl C-R, Heberle J, Kottke T
Indication for a Radical Intermediate Preceding the Signaling State in the LOV Domain Photocycle
Photochem. Photobiol. 87, 548-553 (2011)

Radu I, Schleeger M, Nack M, Heberle J
Time-resolved FT-IR Spectroscopy of Membrane Proteins
Aust. J. Chem. 64, 9-15 (2011)

Krassen H, Ott S, and Heberle J
'In vitro' Hydrogen Production – using Energy from the Sun
Phys. Chem. Chem. Phys., 13, 47-57 (2011)

Thamarath SS, Heberle J, Hore PJ, Kottke T, Matysik J.
Solid-State Photo-CIDNP Effect Observed in Phototropin LOV1-C57S by (13)C Magic-Angle Spinning NMR Spectroscopy.

J. Am. Chem. Soc., 132, 15542-43 (2010)

Jiang, X., Engelhard, M., Ataka, K. & Heberle, J.
‘Molecular Impact of the Membrane Potential on the Regulatory Mechanism of Proton Transfer in Sensory Rhodopsin II  
J. Am. Chem. Soc. 132, 10808-15 (2010)

Ataka, K., Kottke, T. & Heberle, J.
‘Thinner, Smaller, Faster: IR Techniques To Probe the Functionality of Biological and Biomimetic Systems ’ 
Angew. Chem. Int. Ed. 49, 5416 - 5424 (2010)

Ataka, K., Kottke, T.& Heberle, J.
‘Dünner, kleiner, schneller - wie die IR-Spektroskopie zur Aufklärung des Funktionsmechanismus biologischer und biomimetischer Systeme beiträgt ’ 
Angew. Chem. 122, 5544 - 5553 (2010)

Radu I, Budyak IL, Hoomann T, Kim YJ, Engelhard M, Labahn J, Büldt G, Heberle J; Schlesinger R.
‘Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53 ’ 
Biophysical Chemistry 150, 23–28 (2010)

Nack M, Radu I, Gossing M, Bamann C, Bamberg E, von Mollard GF, Heberle J.
‘The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156 ’ 
Photochem Photobiol Sci. 9, 194-198 (2010)

Krassen H, Schwarze A, Friedrich B, Ataka K, Lenz O, Heberle J.
‘Photosynthetic Hydrogen Production by a Hybrid Complex of Photosystem I and [NiFe]-Hydrogenase ’ 
ACS Nano. 3, 4055-4061(2009)


Radu I, Schleeger M, Bolwien C, Heberle J.
‘Time-resolved methods in biophysics. 10. Time-resolved FT-IR difference spectroscopy and the application to membrane proteins ’ 
Photochem Photobiol Sci. 8, 1517-1528 (2009)

Nack, M; Radu; I.; Bamann, C.; Bamberg, E.;Heberle, J.
‘The retinal structure of channelrhodopsin-2 assessed by resonance Raman spectroscopy 
FEBS Letters 583, 3676–3680 (2009)

Bahlawane, N., Ngamou, P.H., Vannier, V., Kottke, T., Heberle, J.& Kohse-Höinghaus, K.
‘Tailoring the properties and the reactivity of the spinel cobalt oxide ’  
PhysChemChemPhys 11, 9224-9232 (2009)

Schleeger, M., Wagner, C., Vellekoop, M., Lendl, B., & Heberle, J.
‘Time-Resolved Flow-Flash FT-IR Difference Spectroscopy: The Kinetics of CO-Photodissociation from Myoglobin Revisited ’  
Anal.Bioanal.Chem.394, 1869-1877 (2009)
An erratum to this article can be found at http://dx.doi.org/10.1007/s00216-009-2928-0

Radu, I., Bamann, C., Nack, M., Nagel, G., Bamberg, E., & Heberle, J.
‘Conformational Changes of Channelrhodopsin-2 ’  
J. Am. Chem. Soc. 131, 7313-7319 (2009)

Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., & Heberle, J.
‘Immobilization of the [FeFe]-Hydrogenase CrHydA1 on a Gold Electrode: Design of a Catalytic Surface for the Production of Molecular Hydrogen ’  
 J. Biotec. 142, 3-9 (2009)

Hiromoto T, Ataka K, Pilak O, Vogt S, Stagni MS, Meyer-Klaucke W, Warkentin E, Thauer RK, Shima S, Ermler U.
‘The crystal structure of C176A mutated [Fe]-hydrogenase suggests an acyl-iron ligation in the active site iron complex. ’
FEBS Lett. 583, 585-590 (2009)

Pfeifer, A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J., & Kottke, T.
‘Time-Resolved Fourier Transform Infrared Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain ’ 
Biophys. J. 96 , 1462-1470 (2009)

Jiang, X., Zaitseva, E., Schmidt, M., Siebert,F., Engelhard, M., Schlesinger, R., Ataka, K., Vogel, R., & Heberle, J.
‘Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy ’  
Proc. Natl. Acad. Sci. USA 105, 12113-12117 (2008)

Ataka, K., Heberle, J.,
‘Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level ’  
Biochem. Soc. Trans. 36, 986-991 (2008)

Jiang, X., Zuber, A., Heberle, J., Ataka, K.
‘ In situ monitoring of the orientated assembly of strep-tagged membrane proteins on the gold surface by surface-enhanced infrared absorption spectroscopy ’
PhysChemChemPhys 10, 6381-6387 (2008)

Jiang, X., Ataka, K. & Heberle, J.,
‘ Influence of the Molecular Structure of Carboxyl Terminated Self-Assembled Monolayer on the Electron Transfer of Cytochrome C Adsorbed on an Au Electrode: in-Situ Observation by Surface Enhanced Infrared Absorption Spectroscopy ’
J. Phys. Chem. C. 112, 813-819 (2008)

Mironova, O. S., Budyak, I. L., Büldt, G., Schlesinger, R., & Heberle, J.,
‘ FT-IR Difference Spectroscopy Elucidates Crucial Interactions of Sensory Rhodopsin I with the Cognate Transducer HtrI. ’,
Biochemistry 46, 9399-9405 (2007)

Majerus, T., Kottke, T., Laan, W., Hellingwerf, K., & Heberle, J.,
‘ Time-Resolved FT-IR Spectroscopy Traces Signal Relay within the Blue-Light Receptor AppA. ’,
ChemPhysChem. 8, 1787-1789 (2007)

Immeln, D., Schlesinger, R., Heberle, J., & Kottke, T.,
‘ Blue Light Induces Radical Formation and Autophosphorylation in the Light-sensitive Domain of Chlamydomonas Cryptochrome. ’,
J. Biol. Chem. 282, 21720–21728 (2007)

Gmelin, W., Zeth, K., Efremov, R., Heberle, J., Tittor, J., & Oesterhelt, D.,
‘ The Crystal Structure of the L1 Intermediate of Halorhodopsin at 1.9 Å Resolution. ’,
Photochem. Photobiol. 83, 369–377 (2007)

Haber-Pohlmeier, S., Abarca-Heidemann, K., Körschen, H.G., Kaur Dhiman, H., Heberle, J., Schwalbe, H., Klein-Seetharaman, J., Kaupp, U.B., & Pohlmeier, A.
‘ Binding of Ca2+ to glutamic acid-rich polypeptides from the rod outer segment. ’,
Biophys. J. 92, 3207–3214 (2007)

Ataka, K., & Heberle, J.,
‘ Biochemical applications of surface-enhanced infrared absorption spectroscopy. ’,
Anal. Bioanal. Chem. 388, 47–54 (2007)

Poetsch, A., Berzborn, R.J., Heberle, J., Link, T.A., Dencher, N.A., & Seelert, H.,
‘ Biophysics and Bioinformatics Reveal Structural Differences of the Two Peripheral Stalk Subunits in Chloroplast ATP Synthase. ’,
J. Biochem. 141, 411–420 (2007)

Badura, A., Esper, B., Ataka, K., Grunwald, C., Wöll, C.,Kuhlmann, J., Heberle, J.& Rögner, M.,
‘ Light-Driven Water Splitting for (Bio-)Hydrogen Production: Photosystem 2 as the Central Part of a Bioelectrochemical Device ’,
Photochem. Photobiol. 82, 1385–1390

Mulkidjanian, A.Y., Heberle, J., & Cherepanov, D.A.,
‘Protons @ interfaces: Implications for biological energy conversion’,
Biochim. Biophys. Acta 1757, 913-930

Hrabakova, J., Ataka, K., Heberle , J., Hildebrandt, P., & Murgida, D.,
‘Long distance electron transfer in cytochrome c oxidase immobilised on electrodes. A surface enhanced resonance Raman spectroscopic study’,
Phys. Chem. Chem. Phys. 8, 759 - 766

Pereira, M.M., Sousa, F.L., Teixeira, M., Nyquist, R.M., & Heberle, J.,
‘A tyrosine residue deprotonates during oxygen reduction by the caa3 reductase from Rhodothermus marinus’,
FEBS Letters 580, 1350–1354

Kottke, T., Ahmad, M., Batschauer, A., & Heberle, J.,
‘Blue-Light-Induced Changes in Arabidopsis Cryptochrome 1 Probed by FTIR Difference Spectroscopy’,
Biochemistry 45, 2472-2479

Ataka, K., Richter, B., & Heberle, J.,
‘Orientational control on the physiological reaction of cytochrome c oxidase tethered to a gold electrode’,
J. Phys. Chem. B. 110, 9339-9347

Kottke, T., Dick, B., Hegemann, P., & Heberle, J.,
‘The Photochemistry of the LOV Domains in the Algal Blue Light Receptor Phot’,
Biopolymers 82, 373-378

Ataka, K., & Heberle, J.,
‘Use of Surface Enhanced Infrared Absorption Spectroscopy (SEIRA) to probe the functionality of a protein monolayer’,
Biopolymers 82, 415-419

Gensch, T., Heberle, J., Viappiani, C. (2006) eds.,
'Special Issue on: ‘Proton transfer in biological systems’,
Photochem Photobiol Sci 5, 529-530

Efremov, R., Gordelyi, V. I., Heberle, J., & Büldt, G.,
‘Time-resolved microspectroscopy on a single crystal of bacteriorhodopsin reveals lattice induced differences in the photocycle kinetics’,
Biophys. J. 91, 1441–1451

Mulkidjanian, A.Y., Cherepanov, D.A., Heberle, J., & Junge, W.,
‘Proton transfer dynamics at membrane/water interface and mechanism of biological energy conversion’,
Biochemistry (Mosc). 70, 251-256

Heberle, J.,
'Vibrational spectroscopy explores the photoreaction of LOV domains'
Light Sensing in Plants (edited by Wada, M., Simazaki, K., & Iino, M.), Springer, Berlin, pp. 155-161

Mironova, O. S., Efremov, R. G., Person, B., Heberle, J.,Budyak, I. L., Büldt, G., & R. Schlesinger,
‘Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli’,
FEBS Lett. 579, 3147-3151

Habuchi, S., Cotlet, M., Gensch, T., Bednarz, T., Haber-Pohlmeier, S., Rozenski, J., Dirix, G., Michiels, J., Vanderleyden, J., Heberle, J., De Schryver, F.C., & Hofkens, J.
‘Cis-trans Isomerization and Decarboxylation in the Photoconversion of the Red Fluorescent Protein DsRed’,
J. Am. Chem. Soc. 127, 8977-8984

Mayer, D., Ataka, K., Heberle, J. & Offenhäusser, A.,
‘Scanning Probe Microscopic Studies of the Oriented Attachment and Membrane Reconstitution of Cytochrome c oxidase to a Gold Electrode,
Langmuir 21, 8580-8583

Heberle, J.,
‘The dynamics of proton transfer across bacteriorhodopsin explored by FT-IR spectroscopy'
Biophysical and Structural Aspects of Bioenergetics (edited by Wikström, M), Royal Society of Chemistry, CambridgeUK, pp. 249-267

Heberle, J., & Ataka, K.,
´SEIDAS: Oberflächenverstärkte IR-Differenzspektroskopie zur funktionellen Untersuchung von Proteinmonolagen,
BIOspektrum 10, 42-44

Nyquist, R.M., Ataka, K., & Heberle, J.,
´The Molecular Mechansism of Membrane Proteins Probed by Evanescent Infrared Waves´,
ChemBioChem 3, 431-436

Bednarz, T., Losi, A., Gärtner, W., Hegemann, P. & Heberle, J.,
‘Functional variations among LOV domains as revealed by FTIR difference spectroscopy’,
Photochem. Photobiol. Sci. 3, 575-579

Ataka, K., & Heberle, J.,
‘Functional vibrational spectroscopy of a cytochrome c monolayer: SEIDAS probes the interaction with different surface modified electrodes’,
J. Am. Chem. Soc. 126, 9445-9457

Heberle, J.,
‘A local area network of protonated water molecules’,
Biophys. J. 87, 2105-2106


Gieß, F., Friedrich, M., Heberle, J., Naumann, R., Knoll, W.,
‘The Protein Tethered Lipid Bilayer – a novel mimic of the biological membrane’,
Biophys. J. 87, 3213-3220

Friedrich, M., Gieß, F., Naumann, R., Knoll, W., Ataka, K., Heberle, J., Hrabakova, J., Murgida, D., & Hildebrandt, P.,
‘Active site structure and redox processes of cytochrome c oxidase immobilised in a novel biomimetic lipid membrane on an electrode’,
Chem. Comm. 21, 2376 –2377

Laan, W., Bednarz, T., Heberle, J., & Hellingwerf, K.,
‘Chromophore composition of a heterologously expressed BLUF-domain’,
Photochem. Photobiol. Sci. 3, 1011-1016

Ataka, K., Giess, F., Knoll, W., Naumann, R., Haber-Pohlmeier, S., Richter, B., & Heberle, J.,
‘Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in-situ monitoring by Surface Enhanced Infrared Absorption Spectroscopy’,
J. Am. Chem. Soc. 126, 16199-16206

Ataka, K., Hegemann, P., & Heberle, J.,
´Vibrational Spectroscopy of an Algal Phot-LOV1 Domain Probes the Molecular Changes Associated with Blue-light Reception´,
Biophys. J. 84, 466-474

Kottke, T., Heberle, J., Hehn, D., Dick, B., & Hegemann, P.,
´Phot LOV1: Photocycle of a Blue Light Receptor Domain from the Green Alga Chlamydomonas reinhardtii´,
Biophys. J. 84, 1192-1201

Gordeliy, V.I., Schlesinger, R., Efremov, R., Büldt, G., Heberle, J.,
´Crystallization in lipidic cubic phases: A case study with Bacteriorhodopsin ´
In: Methods in Molecular Biology Vol. 228: Membrane Protein Protocols: Expression, Purification, and Crystallization (edited by B. Selinsky), Humana Press,
Totowa,NJ, pp. 305-316

Haker, A., Hendriks, J., van Stokkum, I.H.M., Heberle, J., Hellingwerf, K.J.,Crielaard, W., & Gensch, T.,
´The Two Photocycles of Photoactive Yellow Protein from Rhodobacter sphaeroides´,
J. Biol. Chem. 278, 8442-8451

Ataka, K., & Heberle, J.,
´Electrochemically induced Surface Enhanced Infrared Difference Absorption (SEIDA) spectroscopy of a protein monolayer´,
J. Am. Chem. Soc. 125, 4986-4987

Nyquist, R.M., Heitbrink, D., Bolwien, C., Gennis, R.B. & Heberle, J.,
´Direct Observation of Protonation Reactions during the Catalytic Cycle of Cytochrome c Oxidase´,
Proc. Natl. Acad. Sci. USA 100, 8715-8720

Lepke, S., Heberle, J., & Passow, H.,
´The Band 3 Protein: Anion Exchanger and Anion-Proton Cotransporter’ in: Red Cell Membrane Transport in Health and Disease (edited by Bernhardt, I. & Ellory, J.C.), Springer, Berlin, pp. 221-252

Poetsch, A., Rexroth, S., Heberle, J., Link, T.A., Dencher, N.A., & Seelert, H.,
´Characterisation of subunit III and its oligomer from spinach chloroplast ATP synthase´,
Biochim. Biophys. Acta 1618, 59-66

Heitbrink, D., Bolwien, C., Sigurdsson, H., Brzesinski, P. & Heberle, J.,
´Transient binding of CO to CuB in cytochrome c oxidase is dynamically linked to structural changes around a carboxyl group: a time-resolved step-scan Fourier transform infrared investigation´,
Biophys. J. 82, 1-10

Büldt, G., & Heberle, J.,
´Biological Nanomachines´, in: Soft matter: Complex materials on mesocopic scale, Schriften des Forschungszentrums Jülich, Vol. 10, B15

Tittor, J., Paula, S., Subramaniam, S., Heberle, J., Henderson, R. & Oesterhelt, D.,
´Proton translocation by Bacteriorhodopsin in the Absence of Substantial Conformational Changes´,
J. Mol. Biol. 319, 555-565

Friedrich, T., Geibel, S., Ataka, K., Kalmbach, R., Chizov, I., Heberle, J., Engelhard, M., & Bamberg, E.,
´Proteorhodopsin is a Light-driven Proton Pump with Variable Vectoriality´,
J. Mol. Biol. 321, 821-838

Heberle,J., & Gensch, T.,
´When FT-IR spectroscopy meets X-ray crystallography´,
Nature Struct. Biol. 8, 195-197

Zscherp, C., Schlesinger, R., & Heberle, J.,
´Time-resolved FT-IR Spectroscopic Investigation of the pH-dependent Proton Transfer Reactions in the E194Q Mutant of Bacteriorhodopsin´,
Biochem. Biophys. Res. Comm. 283, 57-63

Nyquist, R.M., Heitbrink, D., Bolwien, C., Wells, T.A., Gennis, R.B. & Heberle, J.,
´Perfusion-induced redox differences in cytochrome c oxidase: ATR-FTIR spectroscopy´,
FEBS Lett. 505, 63-67

von Bergen, M., Friedhoff, P., Biernat, J., Heberle, J., Mandelkow, E.-M., Mandelkow, E.
´Assembly of t protein into Alzheimer paired helical filaments depends on a local sequence motif (306VQIVYK311) forming beta structure`,
Proc. Natl. Acad. Sci. USA 97, 5129-5134

Heberle, J. *,
´Proton Transfer Reactions Across Bacteriorhodopsin and Along the Membrane`,
Biochim. Biophys. Acta 1458, 135-147

Heberle, J., Fitter, J., Sass, H.J. & Büldt, G. *,
´Bacteriorhodopsin: The functional details of a molecular machine are being resolved´,
Biophys. Chem. 85, 229-248

Fitter, J. & Heberle, J.,
´Structural equilibrium fluctuations and thermal stability of mesophilic and thermophilic a-amylase´,
Biophys. J. 79, 1629-1636

Zscherp, C., Schlesinger, R., Tittor, J., Oesterhelt, D., & Heberle, J.,
´In-situ Determination of Transient pKa Changes of Internal Amino Acids of Bacteriorhodopsin using Time-Resolved Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy ´,
Proc. Natl. Acad. Sci. USA 96, 5498-5503

Büldt, G., Heberle, J., Schlesinger, R. & Sass, H.J. *,
´Structure, dynamics, and function of the proton pump bacteriorhodopsin´,
in: Methods in Protein Structure Analysis (Ed. Roza, Maria Kamp), Springer Verlag, Berlin, pp. 225-239

Heberle, J. *,
`Time-resolved ATR/FT-IR spectroscopy of Membrane Proteins`,
Recent Research Developments in Applied Spectroscopy (S. G. Pandalai, ed.), Research Signpost, Trivandrum, pp. 147-159

Heberle, J., Büldt, G., Koglin, E., Rosenbusch, J. P., & Landau, E. M.,
´Assessing the Functionality of a Membrane Protein in a Three-Dimensional Crystal´,
J. Mol. Biol. 281, 587-592

Büldt, G., Heberle, J., Dencher, N.A. & Sass, H.J.,
´Structure, dynamics, and function of bacteriorhodopsin´,
J. Protein Chem. 17, 536-538

Zscherp, C., & Heberle, J.,
´Infrared difference spectra of the intermediates L, M, N, and O of the Bacteriorhodopsin photoreaction obtained by time-resolved attenuated total reflection spectroscopy´,
J. Phys. Chem. 101, 10542-10547

Heberle, J., & Zscherp, C.,
´pH-dependence of proton transfer reactions within Bacteriorhodopsin: A time-resolved ATR/FT-IR study´,
in: Spectroscopy of Biological Molecules, Modern Techniques (Ed. P. Carmona, et al.), Kluwer Academic Publishers, Dordrecht, Niederlande, pp. 117-118

Riesle, J., Oesterhelt, D., Dencher, N.A. & Heberle, J.,
´D38 is an essential part of the proton translocation pathway in Bacteriorhodopsin´,
Biochemistry 35, 6635-6643

Heberle, J., & Zscherp, C.,
´ATR/FT-IR difference spectroscopy of biological matter with microsecond time resolution´,
Appl. Spectrosc. 50, 588-596

Zscherp, C., Büldt, G., & Heberle, J.,
´Time-resolved ATR/FT-IR measurements of the Bacteriorhodopsin photocycle´, in: Spectroscopy of Biological Molecules (Ed. J.C. Merlin, et al.), Kluwer Academic Publishers, Dordrecht, Niederlande, pp. 177-178

Hoffmann, A., Hildebrandt, V., Heberle, J. & Büldt, G.,
´Photoactive mitochondria: In vivo transfer of a light-driven proton pump into the inner mitochondrial membrane of Schizosaccharomyces pombe´,
Proc. Natl. Acad. Sci. USA 91, 9367-9371

Heberle, J., Riesle, J., Thiedemann, G., Oesterhelt, D. & Dencher, N.A.,
´Proton migration along the membrane surface and retarded surface to bulk transfer´,
Nature 370, 379-382

Heberle, J., Oesterhelt, D. & Dencher, N.A.,
´Decoupling of photo- and proton cycle in the Asp85Glu mutant of Bacteriorhodopsin´,
EMBO J. 12, 3721-3727

Hildebrandt, V., Fendler, K., Heberle, J., Hoffmann, A., Bamberg, E. & Büldt, G.,
´Bacteriorhodopsin expressed in Schizosaccharomyces pombe pumps protons through the plasma membrane´,
Proc. Natl. Acad. Sci. USA 90, 3578-3582

Heberle, J. & Dencher, N.A.,
´Surface-bound optical probes monitor proton translocation and surface potential changes during the Bacteriorhodopsin photocycle´,
Proc. Natl. Acad. Sci. USA 89, 5996-6000

Heberle, J. & Dencher, N.A.,
´Proton transfer in the light-harvesting membrane protein bacteriorhodopsin - An investigation with optical pH-indicators´,
in: Proton Transfer in Hydrogen-Bonded Systems (Ed. T. Bountis), NATO ASI Series B Physics, Vol. 291, Plenum Press, New York, pp. 187-197

Heberle J. & Dencher, N.A.,
´The surface of the purple membrane: A transient pool for protons ?´,
in: Structures and Functions of Retinal Proteins (Ed. J.L. Rigaud), Colloque INSERM/John Libbey Eurotext Ltd., Vol. 221, pp. 221-224

Dencher, N.A., Büldt, G., Heberle, J., Höltje, H.-D. & Höltje, M.,
´Light-triggered opening and closing of an hydrophobic gate controls vectorial proton transfer across Bacteriorhodopsin´,
in: Proton Transfer in Hydrogen-Bonded Systems (Ed. T. Bountis), NATO ASI Series B Physics, Vol. 291, Plenum Press, New York, pp. 171-185

Dencher, N.A., Heberle, J., Büldt, G., Höltje, H.-D. & Höltje, M.,
´What do neutrons, X-ray synchrotron radiation, optical pH-indicators, and mutagenesis tell us about the light-driven proton pump Bacteriorhodopsin ?´,
in: Membrane Proteins: Structures, Interactions and Models (Eds. Pullmann et al.), Kluwer Academic Publishers, Dordrecht, Niederlande, pp. 69-84

Dencher, N.A., Heberle, J., Büldt, G., Höltje, H.-D. & Höltje, M.,
´Active and passive proton transfer steps through Bacteriorhodopsin are controlled by a light-triggered hydrophobic gate´,
in: Structures and Functions of Retinal Proteins (Ed. J.L. Rigaud), Colloque INSERM/John Libbey Eurotext Ltd.Vol. 221, pp. 213-216

Thiedemann, G., Heberle, J. & Dencher, N.A.,
´Bacteriorhodopsin pump activity at reduced humidity´,
in: Structures and Functions of Retinal Proteins (Ed. J.L. Rigaud), Colloque INSERM/John Libbey Eurotext Ltd., Vol. 221, pp. 217-220

Dencher, N.A., Heberle, J., Bark, C., Koch, M.H.J., Rapp, G., Oesterhelt, D., Bartels, K. & Büldt, G.,
´Proton translocation and conformational changes during the Bacteriorhodopsin photocycle: Time-resolved studies with membrane-bound optical probes and X-ray diffraction´,
Photochem. Photobiol. 54, 881-887

Heberle, J. & Dencher, N.A. (1990),
´Bacteriorhodopsin in ice - Accelerated proton transfer from the purple membrane surface´,
FEBS Lett. 277, 277-280