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Advanced Laboratory Course in Biophysics

Praktikum (20 239) - 4-stündig, ECTS: 10

Dozenten: Joachim Heberle
Sprache: Englisch
Zeitraum: 12.04.2012 bis 12.07.2012
Haupttermin Donnerstag 10 -20 Uhr, Treffen im Raum: 1.1.33
Terminhinweis: 13.04. 10 - 12 am
Introduction to the practical (administration) as part of the lecture.
19.04. 10 - 12 am
MANDATORY introduction to lab and LASER safety.
Also, DEADLINE for attendance of practical.
Contact: svenstripp@zedat.fu-berlin.de


Experiments of the practical course:

(1) Biomolecules at solid-liquid interfaces

    (a) Cyclic voltammetry

    (b) Quartz crystal microbalance


(2) Vibrational spectroscopy of proteins

    (a) FT-IR-difference spectroscopy on proteins

    (b) RAMAN- and resonance RAMAN spectroscopy on proteins


(3) Time-resolved spectroscopy of protein function

    (a) UV/Vis flash photolysis of the photocycle and function of proteins

    (b) Step-scan FT-IR difference spectroscopy of the photocycle of proteins


(4) Thermal and elastic properties of biomembranes

    (a) Thermal phase transition of lipid membranes

    (b) Atomic Force Microscopy and Spectroscopy of a protein film