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Advanced Biophysics 2021

20114101 VORLESUNG

Lecturer: Joachim Heberle, Stephan Block
Language: English
Period: -
Dates: Tue 12:00-14:00, Fri 12:00-14:00 - online

max. number of participants

Notes: 10 ECTS only together with practical course 20114130.
Content: This module will present and substantiate biophysical methods and concepts. Selected methods like spectroscopy and diffraction and their application to proteins, nucleic acids and biomembranes are of particular relevance. The lecture series will cover a selection of the following methods: absorption spectroscopy in the UV, visible and IR region; fluorescence spectroscopy, time-resolved approaches; spectroscopy with linear and circular polarized light; vibrational spectroscopy: Fourier-transform infrared (FTIR), resonance Raman, surfance-enhanced Raman and IR; diffraction with X-rays, Neutrons and electrons; crystallization and protein crystallography; nuclear magnetic resonance (NMR); light scattering; single molecule spectroscopy; optical tweezer; atomic force microscopy; theoretical methods: MD simulations, Poisson-Boltzmann, QM/MM
Literature: Since a comprehensive textbook in Biophysics is not available, here is a list of books from which parts will be used in the lecture: Serdyuk, I.N., Zaccai, N.R. & Zaccai, J: Methods in Molecular Biophysics. Tuszynski & Kurzynski: Introduction to Molecular Biophysics. Cantor & Schimmel: Biophysical Chemistry. Walla: Modern Biophysical Chemistry. Brandén & Tooze: Introduction to Protein Structure. Winter & Noll: Methoden der Biophysikalischen Chemie. Gennis: Biomembranes
Contact: joachim.heberle@fu-berlin.de, stephan.block@fu-berlin.de

Lecture Slides

Lecture 01a - Introduction (Heberle)

Lecture 01b - Cells & Microscopy (Block)

Lecture 02a - Fluorescence Microscopy Part I (Block)

Lecture 02b - Fluorescence Microscopy Part II (Block)

Lecture 03a - Electron Microscopy Part I (Block)

Lecture 03b - Electron Microscopy Part II (Block)

Lecture 04a - Scanning Probe Approaches Part I (Block)

Lecture 04b - Scanning Probe Approaches Part II (Block)

Lecture 05a - Scanning Probe Approaches Part III (Block)

Lecture 05b - Amino Acids (Block)

Lecture 06a - Protein Structure (Block)

Lecture 06b - Co-Factors and Protein Structure Determination (Block)

Lecture 07a - X-ray Crystallography: Concepts (Block)

Lecture 07b - X-ray Crystallography: Synchrotron Radiation (Block)

Lecture 08a - X-ray Free Electron Laser (Block)

Lecture 08b - Protein Crystallization (Block)